THE ALU FAMILY REPEAT PROMOTER HAS A TRANSFER RNA-LIKE BIPARTITE STRUCTURE
THE ALU FAMILY REPEAT PROMOTER HAS A TRANSFER RNA-LIKE BIPARTITE STRUCTURE
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DOI：
10.1002/j.1460-2075.1983.tb01486.x
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发表时间：
1983-01-01
期刊：
影响因子：
11.4
通讯作者：
BARALLE, FE
中科院分区：
文献类型：
--
作者：
PAOLELLA, G;LUCERO, MA;BARALLE, FE
绘制了RNA聚合酶III进行Alu重复转录所需的[人类]基本序列。实验证据表明，Alu重复启动子是组织在一个二分结构类似的分裂tRNA启动子所建议的DNA序列同源性。通过在几个重组克隆中组合来自高效和低效转录的天然Alu重复序列的片段，有可能研究导致其差异的区域。除了与tRNA共有序列的短段同源性外，启动子序列中几乎没有限制。对转录效率的一些影响可由可接受的启动子组分之外的区域施加。
The [human] essential sequences needed for Alu repeat transcription by RNA polymerase III were mapped. Experimental evidence is presented showing that the Alu repeat promoters are organized in a bipartite structure similar to the split tRNA promoters as suggested by DNA sequence homology. By combining fragments from efficiently and inefficiently transcribed natural Alu repeats in several recombinant clones, it was possible to investigate the regions responsible for their differences. Apart from the short stretches of homology with the tRNA consensus sequence, there is very little constraint in the promoter sequences. Some influence on the efficiency of transcription may be exerted by regions outside the accepted promoter components.