Antibody binding of cartilage-specific proteoglycans.
Antibody binding of cartilage-specific proteoglycans.
复制标题
软骨特异性蛋白多糖的抗体结合。
DOI：
10.1016/0003-9861(80)90316-1
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发表时间：
1980
影响因子：
3.9
通讯作者：
P. Goetinck
中科院分区：
文献类型：
--
作者：
K. Sparks;P. L. Lever;P. Goetinck
来自鸡的软骨特异性蛋白聚糖的抗血清含有结合胚胎[35 S]硫酸盐标记的软骨特异性蛋白聚糖（[35 S]PGS I）的IgG。抗体与[35 S]PGS I的结合不需要预先用睾丸透明质酸酶或软骨素酶ABC消化抗原。使用Farr的沉淀法证明了抗原-抗体反应产物中存在[35 S]PGS I，并通过证明山羊抗兔IgG血清可沉淀兔抗PGS I-[35 S]PGS I复合物而不是单独的[35 S]PGS I来证实。该抗体可以结合基本上所有（>90%）的[35 S]PGS I。抗体可以单体或聚集形式结合[35 S] PGSI。该抗血清不含针对许多糖胺聚糖、来自皮肤的蛋白聚糖或软骨的次要蛋白聚糖组分（PGS II）的抗体。抗血清交叉反应软骨特异性蛋白聚糖从不同的鸟类属，日本鹌鹑。
Antiserum to cartilage-specific proteoglyean from chickens contains IgG which binds embryonic [35S]sulfate-labeled cartilage-specific proteoglyean ([35S]PGS I). The binding of antibody to [35S]PGS I does not require prior digestion of the antigen with testicular hyaluronidase or chondroitinase ABC. Presence of [35S]PGS I in the antigen-antibody reaction product was demonstrated using the precipitation method of Farr and confirmed by demonstrating that goat anti-rabbit IgG serum can precipitate rabbit anti PGS I—[35S]PGS I complexes but not [35S]PGS I alone. The antibody can bind essentially all (>90%) of the [35S]PGS I. Antibody can bind to [35S]PGS I in either the monomeric or the aggregated form. The antiserum does not contain antibodies to a number of glycosaminoglycans, to proteoglyean from skin or to a minor proteoglyean component of cartilage (PGS II). The antiserum cross-reacts with cartilage-specific proteoglycan from a different avian genus, the Japanese quail.