Immuohistochemical markers for pituicyte
Immuohistochemical markers for pituicyte
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DOI：
10.1016/j.neulet.2009.06.059
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发表时间：
2009-11
影响因子：
2.5
通讯作者：
Xiaoyan Wei;Cai-hong Zhao;Yingying Liu;Ya-zhou Wang;G. Ju
中科院分区：
文献类型：
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作者：
Xiaoyan Wei;Cai-hong Zhao;Yingying Liu;Ya-zhou Wang;G. Ju
垂体细胞是一种特殊的星形胶质细胞，GFAP一直被用作垂体细胞的特异性标志物。GFAP和S100β是两种常用的星形胶质细胞标志物。它们的免疫反应在大脑的不同区域是不同的。据我们所知，这一问题尚未在垂体细胞中研究过。在我们的初步研究中，我们发现抗GFAP和S100β的抗体对垂体细胞的染色不同。因此，在大鼠身上进行了光镜和电镜技术的详细研究。光镜下，抗gfap和抗s100 β染色率分别为66.78%和86.78%。在超微结构水平上发现这种差异是细胞类型特异性的。垂体实质细胞均可见GFAP和S100β抗体，而纤维性垂体细胞仅具有S100β免疫反应。这种细胞类型特异性的功能意义仍有待阐明。
GFAP has long been adopted as the specific marker for pituicyte, a special type of astrocyte. GFAP and S100β are two commonly used astrocyte markers. Their immunoreactivities differ in different regions of the brain. To our knowledge this issue has not been studied in pituicyte. In our preliminary study, we found that antibodies against GFAP and S100β stained the pituicytes differently. A detailed investigation with both light and electron microscopic techniques was thus conducted in the rat. At light microscopic level, anti-GFAP and anti-S100β stained 66.78% and 86.78% of the pituicytes, respectively. It was found at ultrastructural level that this difference was cell type specific. The parenchymatous pituicytes could be stained with antibodies against both GFAP and S100β, whereas the fibrous pituicytes were only S100β-immunoreactive. The functional significance of this cell type specificity remains to be elucidated.