Effects of adrenalectomy on CART expression in the rat arcuate nucleus
Effects of adrenalectomy on CART expression in the rat arcuate nucleus
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DOI：
10.1002/syn.10213
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发表时间：
2003-10-01
期刊：
影响因子：
2.3
通讯作者：
Kuhar, MJ
中科院分区：
文献类型：
--
作者：
Balkan, B;Koylu, E;Kuhar, MJ
为了测试弓状核中CART的糖皮质激素调节，在大鼠组中进行肾上腺切除术（ADX）和激素替代（HRs）。CART mRNA水平通过原位杂交测定，CART肽水平通过免疫细胞化学测定。ADX导致弓形体CART mRNA和肽水平降低，这被HR逆转。这些结果表明糖皮质激素调节弓形体CART。这种调节可以通过糖皮质激素受体的作用直接进行，也可以通过ADX诱导的瘦素水平变化间接进行。这些发现表明CART在应激反应中的作用。(C)2003 Wiley-Liss，Inc.
In order to test for glucocorticoid regulation of CART in the arcuate nucleus, adrenalectomies (ADX) and hormone replacements (HRs) were carried out in groups of rats. CART mRNA levels were determined by in situ hybridization and CART peptide levels by immunocytochemistry. ADX caused a lowering of CART mRNA and peptides levels in the arcuate and this was reversed by HR. These results indicate a glucocorticoid regulation of CART in the arcuate. The regulation could be direct through an action of glucocorticoid receptors or indirectly through ADX-induced changes in leptin levels. These findings suggest a role for CART in the stress response. (C) 2003 Wiley-Liss, Inc.