Mitochondrial DNA sequence variation among geographic strains of diamondback moth (Lepidoptera: Plutellidae)
Mitochondrial DNA sequence variation among geographic strains of diamondback moth (Lepidoptera: Plutellidae)
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DOI：
10.1093/aesa/90.5.590
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发表时间：
1997-09-01
影响因子：
2.3
通讯作者：
Roderick, GK
中科院分区：
文献类型：
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作者：
Chang, WXZ;Tabashnik, BE;Roderick, GK
我们使用编码细胞色素氧化酶 I (COI) 的线粒体基因的 365 个碱基对检查了 6 个小菜蛾地理品系 Plutella xylostella (L.) 之间的遗传变异。 6 个菌株中的 5 个菌株中未检测到序列变异； 1 个菌株包含 2 个单倍型，其差异在于单碱基替换 (0.27%)。来自夏威夷、菲律宾和宾夕法尼亚州的小菜蛾品系之间的序列差异范围为 0% 至 0.82%。除了一个例外，品系之间的碱基对替换导致同义密码子，并且不会改变氨基酸序列。小菜蛾品系之间的遗传分化与每个品系来源之间的地理距离不相关。小菜蛾种群之间的 COI 变异与其他鳞翅目物种中发现的变异相似，并且远小于小菜蛾和 2 个未描述的夏威夷小菜蛾物种之间的变异（分别为 5% 和 10%）。这里报告的序列为相关工作提供了基准数据，例如分析小菜蛾对苏云金芽孢杆菌的全球抗性模式。
We examined genetic variation among 6 geographic strains of diamondback moth, Plutella xylostella (L.), using 365 base pairs of the mitochondrial gene encoding cytochrome oxidase I (COI). No sequence variation was detected within 5 of the 6 strains; 1 strain contained 2 haplotypes that differed by a single base substitution (0.27%). Sequence differences between strains of diamondback moth from Hawaii, the Philippines, and Pennsylvania ranged from 0 to 0.82%. With one exception, base pair substitutions among strains resulted in synonymous codons and did not alter amino acid sequence, Genetic divergence between strains of diamondback moth was not correlated with geographic distances between the sources of each strain. Variation in COI among populations of diamondback moth was similar to that found in other species of Lepidoptera and was substantially less than variation between diamondback moth and 2 undescribed Hawaiian Plutella species (5 and 10%, respectively). The sequences reported here provide benchmark data for related work, such as analysis of global patterns of resistance to Bacillus thuringiensis in diamondback moth.