POLE OF VAGAL NERVE ACTIVITY DURING SUCKLING - EFFECTS ON PLASMA-LEVELS OF OXYTOCIN, PROLACTIN, VIP, SOMATOSTATIN, INSULIN, GLUCAGON, GLUCOSE AND OF MILK SECRETION IN LACTATING RATS
POLE OF VAGAL NERVE ACTIVITY DURING SUCKLING - EFFECTS ON PLASMA-LEVELS OF OXYTOCIN, PROLACTIN, VIP, SOMATOSTATIN, INSULIN, GLUCAGON, GLUCOSE AND OF MILK SECRETION IN LACTATING RATS
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DOI：
10.1111/j.1748-1716.1994.tb09767.x
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发表时间：
1994-08-01
期刊：
影响因子：
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通讯作者：
UVNASMOBERG, K
中科院分区：
文献类型：
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作者：
ERIKSSON, M;BJORKSTRAND, E;UVNASMOBERG, K
本研究的目的是探讨迷走神经活动的作用，催产素，催乳素和胃肠道（GI）激素的释放在哺乳期间，以及在泌乳大鼠的乳汁分泌。因此，我们对切断迷走神经的哺乳期大鼠进行了实验。将动物断头，从非哺乳大鼠和与排乳相关的哺乳大鼠中采集躯干血。采用放射免疫分析技术测定血浆中催产素、催乳素、血管活性肠肽（VIP）、生长抑素、胰岛素、胰高血糖素和血糖水平。此外，在迷走神经切断后7 d记录母体体重以及窝仔体重。正如预期的那样，催产素和催乳素水平上升，在假手术对照组的吮吸。在迷走神经切断动物的吸吮诱导的催产素的增加被阻断，催乳素水平显着下降。血浆VIP水平增加后，在假手术动物哺乳和迷走神经切断术后未能作出反应。与此相反，生长抑素水平显着上升，在假手术大鼠更显着提高迷走神经切断动物。此外，胰岛素而不是胰高血糖素水平增加了哺乳。然而，迷走神经切断术后胰岛素反应持续存在。有趣的是，吮吸之后，迷走神经切断的动物血糖水平降低，但假手术动物没有。在迷走神经切断术后7 d内，迷走神经切断组大鼠进食量和体重均与假手术组大鼠相同。然而，迷走神经切断大鼠的窝仔与对照窝仔相比，体重增加明显较少。本研究结果表明，迷走神经活动对哺乳期催产素、催乳素、血管活性肠肽和生长抑素的释放具有重要作用。此外，迷走神经活动被认为是足够的乳汁分泌的根本重要性，因为迷走神经切断大鼠的窝体重增加小于假手术动物的窝。
The aim of this study was to investigate the role of vagal nerve activity for the release of oxytocin, prolactin and gastrointestinal (GI) hormones during suckling as well as for the secretion of milk in lactating rats. We have therefore performed experiments on vagotomized lactating rats. The animals were decapitated and trunk blood was collected from nonsuckling rats and from suckling rats in connection with milk ejection. Oxytocin, prolactin, vasoactive intestinal polypeptide (VIP), somatostatin, insulin, glucagon and glucose levels in plasma were measured by RIA-technique. In addition, maternal weight as well as the weight of the litters were recorded 7 d after vagotomy. As expected, oxytocin and prolactin levels rose in response to suckling in sham-operated controls. In vagotomized animals the suckling-induced increase of oxytocin was blocked and prolactin levels were significantly decreased. VIP levels in plasma increased following suckling in sham-operated animals and failed to respond after vagotomy. In contrast, somatostatin levels that rose significantly in sham-operated rats were even more significantly raised in vagotomized animals. In addition, insulin but not glucagon levels were increased by suckling. The insulin response, however, persisted after vagotomy. Interestingly, suckling was followed by a lowering of blood-glucose levels in vagotomized, but not in sham-operated animals. The vagotomized rats ate as much and increased in weight as sham-operated rats during the 7 d of vagotomy. The litters of vagotomized rats, however, gained significantly less weight in comparison with control litters. In conclusion, this study shows that vagal nerve activity is of importance for the release of oxytocin, prolactin, vasoactive intestinal polypeptide and somatostatin during suckling. In addition, vagal nerve activity was found to be of fundamental importance for adequate milk secretion, since litters of vagotomized rats increased in weight less than litters of sham-operated operated animals.