A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci
A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci
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DOI：
10.1016/j.ygeno.2004.12.007
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发表时间：
2005-04-01
期刊：
影响因子：
4.4
通讯作者：
Takasuga, A
中科院分区：
文献类型：
--
作者：
Itoh, T;Watanabe, T;Takasuga, A
构建了牛全基因组7000-rad辐射杂交（RH）面板，SUNbRH（7000-rad），以构建高分辨率RH图谱。以Shirakawa-USDA连锁图为骨架构建了包含3216个微卫星的框架图，在此基础上共获得2377条EST序列。由此产生的RH地图提供了基本上完全覆盖整个基因组，1 cR（7000）对应于114 kb，和牛-人比较地图的1716牛基因和序列注释的人类基因组，其中分别覆盖79%和72%的牛和人类基因组。然后，我们将牛RH和比较图谱与BAC指纹信息整合在www.example.com lab/mapping/bovine中，以构建详细的基于BAC的物理图谱，该物理图谱覆盖了BTA 4上肌内脂肪或"大理石花纹"的40 cM数量性状基因座区域。总之，新的高分辨率SUNbRH（7000-rad），比较，Shirakawa-USDA连锁和BAC指纹图谱提供了一套基因组工具，用于精细定位牛的感兴趣区域。(c)2005年爱思唯尔公司All rights reserved.
A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH(7000-rad), was constructed to build a high-resolution RH map. The Shirakawa-USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided essentially complete coverage across the genome, with 1 cR(7000) corresponding to 114 kb, and a cattle-human comparative map of 1716 bovine genes and sequences annotated in the human genome, which covered 79 and 72% of the bovine and human genomes, respectively. We then integrated the bovine RH and comparative maps with BAC fingerprint information in http://www.bcgsc.ca/ lab/mapping/bovine to construct a detailed, BAC-based physical map covering a reported 40-cM quantitative trait locus region for intramuscular fat or "marbling" on BTA 4. In summary, the new, high-resolution SUNbRH(7000-rad), comparative, Shirakawa-USDA linkage, and BAC fingerprint maps provide a set of genomic tools for fine mapping regions of interest in cattle. (c) 2005 Elsevier Inc. All rights reserved.