CD9, but not other tetraspans, associates with the β1 integrin precursor
CD9, but not other tetraspans, associates with the β1 integrin precursor
复制标题
CD9（但不是其他四跨）与 β1 整合素前体相关
DOI：
--
复制
发表时间：
1997
影响因子：
5.4
通讯作者：
C. Boucheix
中科院分区：
文献类型：
--
作者：
E. Rubinstein;V. Poindessous;F. Le Naour;M. Billard;C. Boucheix
tetraspan超家族的分子具有由四个跨膜（TM）结构域分隔的两个不等的胞外结构域。这些分子在细胞表面上彼此缔合并与其他配偶体分子（特别是β 1整联蛋白）缔合。我们现在表明，CD9与β 1整合素的前体（前β 1）相关。这种关联早在代谢标记后15分钟就被检测到，并且布雷菲德菌素A的使用证明它不需要CD9或整联蛋白β 1的高尔基体修饰。这种相互作用的特异性通过以下事实得到证明：其他四链体（CD 63、CD 81和CD 82）不与前β 1相关，CD 9不与未成熟人类组织相容性白细胞抗原I类相关。为了定位负责与β 1整联蛋白结合的CD9区域，我们产生了两个相互嵌合的CD9/CD82分子，其连接点正好位于第三个TM区域之后。与CD82相比，CD9或第四TM结构域或两者的大细胞外环似乎足以以高效率介导与成熟整联蛋白的缔合。相比之下，与前β 1结合需要分子的至少两个区域。位于第二和第三个TM结构域之间的tetraspan超家族共有位点处的CD9突变不会损害前β 1的共沉淀。最后，由于前β 1与钙连接蛋白相关，我们研究了CD9与该分子伴侣分子的可能相关性。CD9与钙连接蛋白的结合独立于其与β 1整联蛋白的结合，表明钙连接蛋白可能参与CD9的加工。
The molecules of the tetraspan superfamily have two unequal extracellular domains separated by four transmembrane (TM) domains. These molecules are associated on the cell surface with each other and with other partner molecules, in particular β1 integrins. We now show that CD9 associates with the precursor of the β1 integrin (preβ1). This association is detected as early as 15 min after metabolic labeling, and the use of Brefeldin A demonstrates that it does not require Golgi modifications of either CD9 or integrin β1. The specificity of this interaction is demonstrated by the fact that other tetraspans, CD63, CD81, and CD82, do not associate with preβ1, and CD9 does not associate with immature human histocompatibility leukocyte antigen class I. In order to localize the region of CD9 responsible for the association with the β1 integrin, we have generated two reciprocal chimeric CD9/CD82 molecules with the junction localized just after the third TM region. The large extracellular loop of CD9 or the fourth TM domain, or both, appear to be sufficient to mediate an association with the mature integrin with a high efficiency, compared to CD82. By contrast, association with preβ1 requires at least two regions of the molecule. Mutation of CD9 at the consensus site of the tetraspan superfamily, localized between the second and the third TM domain, did not impair the co‐precipitation of preβ1. Finally, because preβ1 associates with calnexin, we have investigated a possible association of CD9 with this chaperone molecule. CD9 associates with calnexin independently of its association with the β1 integrin, suggesting that calnexin could be involved in the processing of CD9.
登录
查看更多内容
分离的整联蛋白胞质结构域抑制“由内而外”的信号转导。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen,YP;O'Toole,TE;Shipley,T;Forsyth,J;LaFlamme,SE;Yamada,KM;Shattil,SJ;Ginsberg,MH
通讯作者：
Ginsberg,MH
B 淋巴细胞的 CD19 信号转导复合物。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bradbury,LE;Goldmacher,VS;Tedder,TF
通讯作者：
Tedder,TF
通过转化生长因子-β 调节细胞粘附受体。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Heino,J;Ignotz,RA;Hemler,ME;Crouse,C;Massagué,J
通讯作者：
Massagué,J
一种基于PCR的高效定点突变方法。
影响因子：
2.7
作者：
Chen,B;Przybyla,AE
通讯作者：
Przybyla,AE
影响因子：
3.3
作者：
LEWIS, JM;SCHWARTZ, MA
通讯作者：
SCHWARTZ, MA