Fatty acids increase the circulating levels of oxidative stress factors in mice with diet‐induced obesity via redox changes of albumin
Fatty acids increase the circulating levels of oxidative stress factors in mice with diet‐induced obesity via redox changes of albumin
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DOI：
10.1111/j.1742-4658.2007.05914.x
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发表时间：
2007-08
期刊：
影响因子：
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通讯作者：
M. Yamato;Takeshi Shiba;Masayoshi Yoshida;T. Ide;Naoko Seri;Wataru Kudou;S. Kinugawa;H. Tsutsui
中科院分区：
文献类型：
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作者：
M. Yamato;Takeshi Shiba;Masayoshi Yoshida;T. Ide;Naoko Seri;Wataru Kudou;S. Kinugawa;H. Tsutsui
代谢综合征中游离脂肪酸的血浆浓度增加，并且增加的脂肪酸可能通过诱导氧化应激而导致细胞损伤。本研究旨在确定脂肪酸的增加是否可以改变白蛋白（Cys34）34位的游离巯基并增强高脂饮食诱导的肥胖小鼠中铜-白蛋白复合物的氧化还原循环活性。小鼠以商业正常饮食或高脂饮食和随意饮水喂养3个月。高脂肪饮食喂养的小鼠出现肥胖、高脂血症和高血糖。高脂肪饮食喂养的小鼠血浆脂肪酸/白蛋白比率也显着增加。脂肪酸/白蛋白比率的增加与白蛋白构象变化和巯基氧化有关。此外，抗坏血酸自由基（铜-白蛋白复合物氧化还原循环活性的指标）仅在肥胖小鼠的血浆中检测到，而抗坏血酸的血浆浓度没有改变。高脂饮食组血浆硫代巴比妥酸反应物质显着增加。这些结果表明，高脂肪饮食组血浆脂肪酸增加导致铜-白蛋白复合物的氧化还原循环激活和过度的脂质过氧化。
Plasma concentrations of free fatty acids are increased in metabolic syndrome, and the increased fatty acids may cause cellular damage via the induction of oxidative stress. The present study was designed to determine whether the increase in fatty acids can modify the free sulfhydryl group in position 34 of albumin (Cys34) and enhance the redox‐cycling activity of the copper–albumin complex in high‐fat diet‐induced obese mice. The mice were fed with commercial normal diet or high‐fat diet and water ad libitum for 3 months. The high‐fat diet‐fed mice developed obesity, hyperlipemia, and hyperglycemia. The plasma fatty acid/albumin ratio also significantly increased in high‐fat diet‐fed mice. The increased fatty acid/albumin ratio was associated with conformational changes in albumin and the oxidation of sulfhydryl groups. Moreover, an ascorbic acid radical, an index of redox‐cycling activity of the copper–albumin complex, was detected only in the plasma from obese mice, whereas the plasma concentrations of ascorbic acid were not altered. Plasma thiobarbituric acid reactive substances were significantly increased in the high‐fat diet group. These results indicate that the increased plasma fatty acids in the high‐fat diet group resulted in the activated redox cycling of the copper–albumin complex and excessive lipid peroxidation.