Detection of an RNA-dependent RNA polymerase in mitochondria from a mitovirus-infected isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi
Detection of an RNA-dependent RNA polymerase in mitochondria from a mitovirus-infected isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi
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DOI：
10.1006/viro.1999.0097
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发表时间：
2000-03-15
期刊：
影响因子：
3.7
通讯作者：
Buck, KW
中科院分区：
文献类型：
--
作者：
Cole, TE;Hong, YG;Buck, KW
在感染新乌米O. novoo -uimi有丝分裂病毒6 （OnuMV6）的新乌米蛇孔虫分离株的线粒体中检测到了RNA依赖性RNA聚合酶（RdRp）的活性。反应产物对应于OnuMVG6RNA的双链和单链形式。使用针对保守RdRp区域的抗体进行Western blot分析，在OnuMV6感染的线粒体中检测到约80 kDa的蛋白质，接近OnuMV6 RdRp的预测大小。在未感染的O. novoo -ulmi分离株的线粒体中未检测到RdRp活性或蛋白。这是首次在真菌线粒体中检测到病毒RdRp，结果与在其合成中使用UGA色氨酸密码子一致。(C) 2000年学术出版社。
RNA-dependent RNA polymerase (RdRp) activity has been detected in mitochondria from an isolate of Ophiostoma novo-ulmi infected with O. novo-uimi mitovirus 6 (OnuMV6). The reaction products corresponded to the double-stranded and single-stranded forms of OnuMVG6RNA. Western blot analysis using antibodies raised against a conserved RdRp region has detected a protein of ca. 80 kDa in OnuMV6-infected mitochondria, close to the predicted size of the OnuMV6 RdRp. No RdRp activity or protein was detected in mitochondria from an uninfected O. novo-ulmi isolate. This is the first detection of a virus RdRp in fungal mitochondria and the results are consistent with the use of UGA tryptophan codons in its synthesis. (C) 2000 Academic Press.