Transcriptional analysis of the conidiation pattern shift of the entomopathogenic fungus Metarhizium acridum in response to different nutrients.
Transcriptional analysis of the conidiation pattern shift of the entomopathogenic fungus Metarhizium acridum in response to different nutrients.
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昆虫病原真菌绿僵菌对不同营养物质响应的分生孢子模式转变的转录分析
DOI：
10.1186/s12864-016-2971-0
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发表时间：
2016-08-09
期刊：
影响因子：
4.4
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Wang Z;Jin K;Xia Y
背景：大多数真菌，包括昆虫病原真菌，在不同的培养条件下，例如在含有不同营养物质的培养基中，有两种不同的分生模式，正常和微循环分生。然而，条件模式转变的机制尚不清楚。结果：在本研究中，在蔗糖酵母提取琼脂（SYA）培养基上进行微循环条件下的酸根绿脓杆菌在培养基中添加蔗糖、硝酸盐或磷酸盐后转向正常条件。利用Solexa/Illumina深度测序技术，将营养物质的变化与分生模式转移和转录变化联系起来，获得了分生模式转移文库。对比分析发现，137个基因在正常分生阶段表达上调，436个基因在微循环分生阶段表达上调。减法文库的比较结果显示，与蔗糖诱导、硝酸盐诱导和磷酸盐诱导的分生模式转变相关的基因分别为83个、216个和168个。利用SYA培养基和4种正常培养基培养的M. acridum分离的mRNA，采用多基因串联法对微循环条件下217个特异性表达基因的表达谱进行分析。142个基因在标准SYA培养基上表达上调。在这142个基因中，101个编码假设的蛋白质或功能未知的蛋白质，只有41个基因编码具有假定功能的蛋白质。在这41个基因中，18个与细胞生长有关，10个与细胞增殖有关，3个与细胞周期有关，3个与细胞分化有关，2个与细胞壁合成有关，2个与细胞分裂有关，7个具有其他功能。这些结果表明，霉霉分生模式的改变主要是由于细胞生长和增殖的改变。结论：当培养基中蔗糖、硝酸盐或磷酸盐含量增加时，m.a ridum的分生模式由微循环分生转变为正常分生。分生模式的调控是一个复杂的过程，涉及到霉霉的细胞周期和代谢。本研究为单一营养物诱导分生模式转变的分子机制提供了重要信息。
Background:Most fungi, including entomopathogenic fungi, have two different conidiation patterns, normal and microcycle conidiation, under different culture conditions, eg, in media containing different nutrients. However, the mechanisms underlying the conidiation pattern shift are poorly understood.Results:In this study, Metarhizium acridum undergoing microcycle conidiation on sucrose yeast extract agar (SYA) medium shifted to normal conidiation when the medium was supplemented with sucrose, nitrate, or phosphate. By linking changes in nutrients with the conidiation pattern shift and transcriptional changes, we obtained conidiation pattern shift libraries by Solexa/Illumina deep-sequencing technology. A comparative analysis demonstrated that the expression of 137 genes was up-regulated during the shift to normal conidiation, while the expression of 436 genes was up-regulated at the microcycle conidiation stage. A comparison of subtractive libraries revealed that 83, 216, and 168 genes were related to sucrose-induced, nitrate-induced, and phosphate-induced conidiation pattern shifts, respectively. The expression of 217 genes whose expression was specific to microcycle conidiation was further analyzed by the gene expression profiling via multigene concatemers method using mRNA isolated from M. acridum grown on SYA and the four normal conidiation media. The expression of 142 genes was confirmed to be up-regulated on standard SYA medium. Of these 142 genes, 101 encode hypothetical proteins or proteins of unknown function, and only 41 genes encode proteins with putative functions. Of these 41 genes, 18 are related to cell growth, 10 are related to cell proliferation, three are related to the cell cycle, three are related to cell differentiation, two are related to cell wall synthesis, two are related to cell division, and seven have other functions. These results indicate that the conidiation pattern shift in M. acridum mainly results from changes in cell growth and proliferation.Conclusions:The results indicate that M. acridum shifts conidiation pattern from microcycle conidiation to normal conidiation when there is increased sucrose, nitrate, or phosphate in the medium during microcycle conidiation. The regulation of conidiation patterning is a complex process involving the cell cycle and metabolism of M. acridum. This study provides essential information about the molecular mechanism of the induction of the conidiation pattern shift by single nutrients.
通过多基因串联体进行基因表达谱分析
影响因子：
3.7
作者：
Jin K;Zheng X;Xia Y
通讯作者：
Xia Y