Synthetic mammalian riboswitches based on guanine aptazyme
Synthetic mammalian riboswitches based on guanine aptazyme
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DOI：
10.1039/c2cc33140c
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Yokobayashi, Yohei
中科院分区：
文献类型：
--
作者：
Nomura, Yoko;Kumar, Deepak;Yokobayashi, Yohei
被鸟嘌呤激活的别构锤头状核酶（适体酶）被用来控制哺乳动物的基因表达的顺式和反式。共表达的两种机制不同的核糖开关导致在一个改进的动态范围内的基因表达。
Allosteric hammerhead ribozymes (aptazymes) that are activated by guanine were used to control mammalian gene expression in cis and in trans. Coexpression of the two mechanistically distinct riboswitches resulted in an improved dynamic range of gene expression.