High Capacity Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry: Coupling a Porous Sheathless Interface with Transient-Isotachophoresis
High Capacity Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry: Coupling a Porous Sheathless Interface with Transient-Isotachophoresis
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DOI：
10.1021/ac102159d
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发表时间：
2010-11-15
影响因子：
7.4
通讯作者：
Mayboroda, Oleg A.
中科院分区：
文献类型：
--
作者：
Busnel, Jean-Marc;Schoenmaker, Bart;Mayboroda, Oleg A.
利用多孔针尖的无鞘接口已被用于耦合毛细管电泳和电喷雾电离质谱。首先，使用接口的有效流速已被表征。发现该界面能够产生稳定的喷雾，其流速范围从低于10 nL/min到>340 nL/min，使得其能够用于电喷雾过程的质量或浓度敏感区域。随后，通过分析复杂性不断增加的肽混合物，我们证明了该平台提供了极高的灵敏度，能够以极高的分辨率检测极少量的材料。瞬时等速电泳（t-ITP）也可以与此设置集成，以增加系统的质量负载，同时保持峰值效率和分辨率。在有或没有t-ITP的情况下，可分别达到亚纳摩尔或纳摩尔范围内的检测浓度极限。在分离毛细管的入口处施加真空进一步允许提高系统的峰值容量，同时还提高其效率。作为本研究的最后一步，证明了界面的固有性质允许使用涂覆的不带电表面，以便可以实现非常高的高峰容量。
A sheathless interface making use of a porous tip has been used for coupling capillary electrophoresis and electrospray ionization mass spectrometry. First, effective flow rates using the interface have been characterized. It was found that the interface is capable of generating a stable spray with flow rates ranging from below 10 nL/min to >340 nL/min, enabling its use in either the mass or concentration-sensitive region of the electrospray process. Subsequently, by analyzing peptide mixtures of increasing complexity, we have demonstrated that this platform provides exquisite sensitivity enabling the detection of very low amounts of materials with very high resolving power. Transient isotachophoresis (t-ITP) can also be integrated with this setup to increase the mass loading of the system while maintaining peak efficiency and resolution. Concentration limits of detection in the subnanomolar or nanomolar range can be achieved with or without t-ITP, respectively. The application of a vacuum at the inlet of the separation capillary further allowed the peak capacity of the system to be improved while also enhancing its efficiency. As a final step in this study, it was demonstrated that the intrinsic properties of the interface allows the use of coated noncharged surfaces so that very high peak capacities can be achieved.