Cytoplasmic Protein Imaging with Mid-Infrared Photothermal Microscopy: Cellular Dynamics of Live Neurons and Oligodendrocytes
Cytoplasmic Protein Imaging with Mid-Infrared Photothermal Microscopy: Cellular Dynamics of Live Neurons and Oligodendrocytes
复制标题
DOI：
10.1021/acs.jpclett.9b00616
复制
发表时间：
2019-06-06
影响因子：
5.7
通讯作者：
Cho, Minhaeng
中科院分区：
文献类型：
--
作者：
Lim, Jong Min;Park, Chanjong;Cho, Minhaeng
中红外光热显微镜已被建议作为替代传统的红外显微镜，因为除了固有的化学对比度振动激发时，它可以切实可行地实现亚微米级的空间分辨率。此外，它具有很大的潜力，用于实时生物成像的细胞动力学观察没有光损伤或光漂白的荧光标记。我们对少突胶质细胞进行了实时成像，以研究细胞整个生命周期的细胞动力学，揭示细胞分裂和凋亡以及细胞迁移的细节。在活神经元的情况下，我们观察到光热对比与轴突上的旅行蛋白质复合物，这对应于从细胞体到神经元的树突状分支通过细胞骨架的囊泡的运输。我们预计，中红外光热成像将是非常有用的，以获得深入了解生物物理科学领域，特别是关于细胞动力学和功能。
Mid-infrared photothermal microscopy has been suggested as an alternative to conventional infrared microscopy because in addition to the inherent chemical contrast available upon vibrational excitation, it can feasibly achieve spatial resolution at the submicrometer level. Furthermore, it has substantial potential for real-time bioimaging for the observation of cellular dynamics without photodamage or photobleaching of fluorescent labels. We performed real-time imaging of oligodendrocytes to investigate cellular dynamics throughout the life cycle of a cell, revealing details of cell division and apoptosis, as well as cellular migration. In the case of live neurons, we observed a photothermal contrast associated with traveling protein complexes on an axon, which correspond to the transport of vesicles from the cell body to the dendritic branches of the neuron through the cytoskeleton. We anticipate that mid-infrared photothermal imaging will be of great use for gaining insights into the field of biophysical science, especially with regard to cellular dynamics and functions.