MONOCLONAL-ANTIBODY AGAINST PRAD1/CYCLIN D1 STAINS NUCLEI OF TUMOR-CELLS WITH TRANSLOCATION OR AMPLIFICATION AT BCL-1 LOCUS
MONOCLONAL-ANTIBODY AGAINST PRAD1/CYCLIN D1 STAINS NUCLEI OF TUMOR-CELLS WITH TRANSLOCATION OR AMPLIFICATION AT BCL-1 LOCUS
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DOI：
10.1111/j.1349-7006.1994.tb02969.x
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发表时间：
1994-09-01
期刊：
影响因子：
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通讯作者：
SETO, M
中科院分区：
文献类型：
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作者：
BANNO, S;YOSHIKAWA, K;SETO, M
针对由人PRAD 1/细胞周期蛋白D1基因编码的细菌产物产生小鼠单克隆抗体，该基因已知与11 q13的BCL-1基因座的易位或扩增有关。所用的免疫抗原为GST-PRAD 1和T7基因10-PRAD 1融合产物。在免疫印迹上，四种抗体与PRAD 1融合产物和具有t（11;14）（q13;q32）扩增的B细胞肿瘤细胞系和具有11 q13扩增的乳腺癌细胞系的细胞裂解物均反应。一项免疫荧光研究表明，只有一个染色细胞核与11 q13异常。由于该抗体被证明适用于常规石蜡包埋的组织切片，因此对各种淋巴瘤组织进行免疫组织学染色。11例套细胞淋巴瘤中有8例呈中等至强阳性，6例阳性病例表现出特征性的染色模式，主要是核或核和胞质。在其他类型的淋巴瘤中未观察到核染色模式，因此可能与PRAD 1 mRNA过表达相关。
Mouse monoclonal antibodies were produced against the bacterial product encoded by human PRAD1/cyclin D1 gene, which is known to be involved in tumors with translocation or amplification at BCL-1 locus of 11q13. The immunizing antigens used were GST-PRAD1 and T7 gene 10-PRAD1 fusion products. Four antibodies were reactive with both PRAD1 fusion products and cell lysates of B-cell tumor cell lines with t(11;14)(q13;q32) and a breast cancer cell line with 11q13 amplification, on immunoblotting. An immunofluorescence study showed that only one of them stained nuclei of cells with 11q13 abnormalities. Since this antibody proved applicable for conventional paraffin-embedded tissue sections, immunohistologic staining of various lymphoma tissues was performed. Eight of 11 mantle cell lymphomas showed intermediate to strong positivity and 6 of the positive cases demonstrated characteristic staining patterns that were either predominantly nuclear or both nuclear and cytoplasmic. The nuclear staining pattern was not observed with other types of lymphoma and thus may correlate with PRAD1 mRNA overexpression.