PRESENCE AND DISTRIBUTION OF HUMAN PAPILLOMAVIRUS SENSE AND ANTISENSE RNA TRANSCRIPTS IN GENITAL CANCERS
PRESENCE AND DISTRIBUTION OF HUMAN PAPILLOMAVIRUS SENSE AND ANTISENSE RNA TRANSCRIPTS IN GENITAL CANCERS
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DOI：
10.1099/0022-1317-72-4-885
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发表时间：
1991-04-01
影响因子：
3.8
通讯作者：
BURRELL, CJ
中科院分区：
文献类型：
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作者：
HIGGINS, GD;UZELIN, DM;BURRELL, CJ
用I-125标记的亚基因组核探针原位杂交技术，分析了8例HPV-16阳性生殖器癌和2株宫颈鳞癌(SCC)来源的细胞系中的RNA转录。E6和E7开放阅读框对应的转录本总是存在，除了分化的鳞状细胞角化层内。在两个细胞系和一些肿瘤中都能检测到核内的E1基因转录本，而在七个可评估的肿瘤中有五个没有检测到E2/E4转录本，这表明转录来自整合的病毒DNA。当以mRNA正义核糖核酸酶探针为对照时，两种细胞系均未见信号；然而，使用mRNA正义核糖核糖核酸酶探针，三个肿瘤细胞含有一个焦点、强烈的核核糖核酸酶敏感信号。这种反义RNA信号映射到包括非编码区在内的整个基因组中。在一个肿瘤中，在核糖核酸酶保护试验中，使用两个方向的E7和E2/E4RNA探针独立地证实了反义RNA的存在。反义RNA的转录可能是一些HPV阳性生殖器肿瘤的自然特征，其在体内调节细胞行为的可能作用需要进一步研究。
RNA transcription in eight human papillomavirus (HPV) type 16-positive genital carcinomas and in two cervical squamous cell carcinoma (SCC)-derived cell lines was analysed by in situ hybridization using I-125-labelled subgenomic riboprobes. Transcripts corresponding to the E6 and E7 open reading frames were always present, except within the keratinizing layers of differentiated SCCs. Intranuclear E1 gene transcripts were detectable in both cell lines and some tumours whereas E2/E4 transcripts were absent from five of seven assessable tumours, suggesting transcription from integrated viral DNA. When mRNA sense riboprobes were used as controls, no signal was seen in the two cell lines; however, three tumours contained a focal, intense nuclear RNase-sensitive signal using mRNA sense riboprobes. This antisense RNA signal mapped across the whole genome including the non-coding region. In one tumour, the presence of antisense RNA was independently confirmed by using E7 and E2/E4 RNA probes of both orientations in RNase protection assays. Transcription of antisense RNA may be a natural feature of some HPV-positive genital tumours and its possible role in modulating cell behaviour in vivo requires further investigation.