Two types of collecting duct mitochondria-rich (intercalated) cells: lectin and band 3 cytochemistry.
Two types of collecting duct mitochondria-rich (intercalated) cells: lectin and band 3 cytochemistry.
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两种类型的集合管富含线粒体（嵌入）的细胞：凝集素和带 3 细胞化学。
DOI：
10.1152/ajpcell.1986.251.3.c347
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Jennings,ML
中科院分区：
文献类型：
--
作者：
Schuster,VL;Bonsib,SM;Jennings,ML
阴离子交换在肾离子转运和酸化中起重要作用。为了进一步了解肾上皮阴离子交换的分子性质，我们使用了一种单克隆抗体的膜结构域（52 kDa）的人红细胞带3蛋白免疫细胞化学搜索这种多肽在兔肾。在冷冻切片中，皮质和外髓集合管中的细胞亚群显示出免疫反应性;标记仅限于基底外侧膜。近端小管和Henle粗肢和细肢均无免疫反应性。皮质中大约11%的细胞和髓质集合管中43%的细胞呈第3带阳性。为了确定带3阳性的细胞类型，通过其琥珀酸脱氢酶活性的阳性组织化学染色和其在顶膜结合花生凝集素的能力来鉴定富含胡萝卜素（插入）的细胞。在皮质集合小管中，大多数富含花生凝集素的细胞结合花生凝集素，但带3阴性;其余的带3阳性，但凝集素阴性。这种分布在外髓的内纹中是相反的：所有富含维生素A的细胞均为带3阳性和凝集素阴性。因此，富含钙的细胞至少有两种类型，每种类型都有不同的轴向分布模式。考虑到兔集合小管体外HCO 3转运特性的现有信息，可能是凝集素阳性、带3阴性的富含HCO 3的细胞分泌HCO 3，而凝集素阴性、带3阳性的细胞重吸收HCO 3（分泌H）。
Anion exchange plays an important role in renal ion transport and acidification. To further understand the molecular nature of renal epithelial anion exchange, we used a monoclonal antibody to the membrane domain (52 kDa) of human erythrocyte band 3 protein to immunocytochemically search for this polypeptide in the rabbit kidney. In cryostat sections, a subpopulation of cells in the cortical and outer medullary collecting tubules showed immunoreactivity; labeling was restricted to the basolateral membrane. Proximal tubules and thick and thin limbs of Henle showed no immunoreactivity. Approximately 11% of cells in the cortical, but 43% of cells in the medullary, collecting tubule were positive for band 3. To determine the type of cells that were band 3 positive, mitochondria-rich (intercalated) cells were identified by their positive histochemical staining for succinic dehydrogenase activity and by their ability to bind peanut lectin at the apical membrane. In the cortical collecting tubule, the majority of mitochondria-rich cells bound peanut lectin but were band 3 negative; the remainder were band 3 positive but lectin negative. This distribution was reversed in the inner stripe of the outer medulla: all mitochondria-rich cells were band 3 positive and lectin negative. Thus mitochondria-rich cells are of at least two types, each of which has a distinct axial distribution pattern. Given available information about in vitro HCO3 transport properties of rabbit collecting tubules, it is likely that the lectin-positive, band 3-negative mitochondria-rich cells secrete HCO3, whereas the lectin-negative, band 3-positive cells reabsorb HCO3 (secrete H).