A model of low-temperature water transport for hepatocyte spheroids.
A model of low-temperature water transport for hepatocyte spheroids.
复制标题
肝细胞球体低温水运输模型。
DOI：
10.1111/j.1749-6632.1998.tb10152.x
复制
发表时间：
1998
影响因子：
5.2
通讯作者：
Hubel,A
中科院分区：
文献类型：
--
作者：
Korniski,B;Hubel,A
Spheroids are multicellular aggregates that exhibit a more tissue‐like morphology and function when compared to monolayer cultures of the same cells. Hepatocyte spheroids are presently under investigation for use in an artificial liver. The ability to cryopreserve hepatocyte spheroids is essential for their clinical and commercial application. A multicompartment model was formulated to predict water content as a function of temperature during freezing. The theoretical predictions of water transport indicate that there will be spatial differences in water content of the spheroid during freezing and that due to the rapid decrease in water transport with decreasing temperature, the undercooling of the intracellular solution during freezing will increase steadily. These results indicate that conventional freezing of hepatocyte spheroids will be difficult to accomplish due to transport limitations in the spheroids.
