Expression of prostate-specific membrane antigen in normal and malignant human tissues
Expression of prostate-specific membrane antigen in normal and malignant human tissues
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DOI：
10.1007/s00268-005-0544-5
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发表时间：
2006-04-01
影响因子：
2.6
通讯作者：
Haas, GP
中科院分区：
文献类型：
--
作者：
Kinoshita, Y;Kuratsukuri, K;Haas, GP
背景：前列腺特异性膜抗原（PSMA）在雄激素依赖性前列腺癌中表达上调，并且已成为该癌症免疫治疗和诊断的靶点。然而，这种蛋白质也在其他组织中表达。本研究的目的是调查其在正常和恶性人体组织中的表达情况。
方法：使用单克隆抗体24.4E6（对638 - 657位氨基酸残基具有特异性）和7E11.C5（对PSMA的跨膜结构域具有特异性），对手术标本进行PSMA的免疫组化检测。
结果：在前列腺上皮、膀胱、肾近端小管、肝脏、食管、胃、小肠、结肠、乳腺、输卵管和睾丸生精小管、海马神经元和星形胶质细胞、室管膜、肾上腺皮质和髓质以及卵巢间质中检测到前列腺特异性膜抗原。在前列腺、肾、膀胱、胃、小肠、结肠、肺、肾上腺和睾丸的肿瘤中也检测到该抗原。在正常精囊或肺中未检测到。
结论：这些发现表明PSMA广泛分布于正常组织中，并且根据肿瘤的不同，其表达上调、下调或不变。PSMA的广泛分布可能使其适用于多种肿瘤的诊断和治疗。
Background: Prostate-specific membrane antigen (PSMA) is upregulated in androgen-dependent prostate carcinoma and it has been targeted for immunotherapy and diagnosis of this cancer. However, this protein is also expressed in other tissues. The objective of this study is to investigate its expression in normal and malignant human tissues.Methods: Using monoclonal antibodies 24.4E6 (specific for residues 638-657) and 7E11.C5 (specific for the transmembrane domain of PSMA), immunohistochemical detection of PSMA was performed in surgical specimens.Results: Prostate-specific membrane antigen was detected in the epithelium of prostate, urinary bladder, proximal tubules of kidney, liver, esophagus, stomach, small intestine, colon, breast, fallopian tubes and testicular seminiferous tubules, hippocampal neurons and astrocytes, ependyma, cortex and medulla of the adrenal gland, and ovary stroma. It was also detected in neoplasms of the prostate, kidney, urinary bladder, stomach, small intestine, colon, lung, adrenal gland, and testis. It was not detected in normal seminal vesicles or the lung.Conclusions: These findings demonstrate that PSMA is widely distributed in normal tissues, and, depending on the tumors, its expression is up- or down-regulated, or unchanged. The broad distribution of PSMA may make it suitable for the diagnosis and therapy of a wide variety of tumors.