High-throughput verification of transcriptional starting sites by Deep-RACE
High-throughput verification of transcriptional starting sites by Deep-RACE
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DOI：
10.2144/000113066
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发表时间：
2009-02-01
期刊：
影响因子：
2.7
通讯作者：
Carninci, Piero
中科院分区：
文献类型：
--
作者：
Olivarius, Signe;Plessy, Charles;Carninci, Piero
我们提出了一种高通量的方法来研究感兴趣的基因的转录起始位点，我们命名为Deep-RACE（cDNA末端的深度快速扩增）。利用最新的测序技术，它可以并行分析多个基因，并且无需耗时的克隆步骤。与RACE PCR产物的测序相比，我们的方法更精确，甚至对于小到17个的批次也更具成本效益。
We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes an is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.