Differentiation and quality control of smooth muscle cells from human pluripotent stem cells via the neural crest lineage
Differentiation and quality control of smooth muscle cells from human pluripotent stem cells via the neural crest lineage
复制标题
通过神经嵴谱系从人多能干细胞中分化平滑肌细胞并进行质量控制
DOI：
10.1101/2023.05.31.543049
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Holt P
中科院分区：
文献类型：
--
作者：
Holt P
Sinha实验室已经开发了沿着发育谱系特异性途径将人类多能干细胞（hPSCs）分化为血管平滑肌细胞的方案。在发育过程中，旁轴中胚层（PM）、侧板中胚层（LM）和神经嵴（NC）谱系都以特定位置的方式产生平滑肌细胞。来自患病患者的诱导PSCs为在实验室中建立疾病相关细胞模型提供了一个平台。在这里，我们描述了一种通过神经嵴谱系将hPSCs分化为血管平滑肌细胞的稳健方案，以及确保持续高质量分化细胞所需的控制步骤。
The Sinha laboratory has developed protocols for differentiating human pluripotent stem cells (hPSCs) into vascular smooth muscle cells along developmental lineage-specific pathways. In development, paraxial mesoderm (PM), lateral plate mesoderm (LM) and neural crest (NC) linages each give rise to smooth muscle cells significant in a location-specific manner. Induced PSCs derived from patients enduring disease provide a platform from which disease-relevant cell models can be established in the laboratory. Here we describe a robust protocol for differentiating hPSCs into vascular smooth muscle cells via a neural crest lineage and the control steps required to ensure consistently high-quality differentiated cells.