Amplification primers that target locus-specific introns in actinopterygian fishes
Amplification primers that target locus-specific introns in actinopterygian fishes
复制标题
作者：
Quattro, JM;Jones, WJ
种间和种内系统发育的研究得益于针对线粒体基因组特定区域的“通用”扩增引物的开发。不幸的是，类似的针对核编码区的引物组并不总是被证明是可靠的，在针对各种生物体的单个基因座上。我们描述了一种策略，用于开发一组寡核苷酸引物，其侧翼位于单个基因座内保守的内含子位置。我们通过开发两套针对大量放线蕨类鱼类的多态、单基因座内含子区域的扩增引物来证明这一方法。
The study of interspecific and intraspecific phylogeny has benefited from the development "universal" amplification primers that target specific regions of the mitochondrial genome. Unfortunately, analogous primer sets for nuclear-encoded regions have not always proven reliable in targeting single loci across a wide variety of organisms. We describe a strategy for developing sets of oligonucleotide primers that flank conserved intron positions within single loci. We demonstrate this approach through development of two sets of amplification primers that target polymorphic, single-locus intron regions from a large number of actinopterygian fishes.