Evaluation of recombinant antigens of Trypanosoma cruzi to diagnose infection in naturally infected dogs from Chaco region, Argentina
Evaluation of recombinant antigens of Trypanosoma cruzi to diagnose infection in naturally infected dogs from Chaco region, Argentina
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DOI：
10.1111/pim.12144
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发表时间：
2014-12-01
影响因子：
2.2
通讯作者：
Cimino, R. O.
中科院分区：
文献类型：
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作者：
Floridia-Yapur, N.;Vega-Benedetti, A. F.;Cimino, R. O.
狗被认为是哺乳动物中克氏锥虫的主要宿主。因此，准确检测犬群中的克氏锥虫感染具有流行病学意义。在此，我们以ELISA形式分析了克氏锥虫重组抗原FRA、SAPA、CP 1、Ag 1和SAPA/TSSA VI混合物的效用。我们使用了从阿根廷查科地区的38只狗获得的血清的阳性对照组，其具有阳性匀浆-ELISA反应，所有这些狗也通过异种诊断和/或PCR呈阳性。阴性组包括来自非流行区的19只犬。比较两种方法的敏感性、特异性、受试者工作特征曲线（ROC）曲线下面积（AUC）和Kappa指数。SAPA/TSSA VI具有最高的性能，灵敏度为947%，AUC ROC为099，表明准确性高。在单个抗原中，SAPA-ELISA产生了最高的灵敏度（868%）和AUC ROC（096），而FRA-ELISA是效率最低的测试（灵敏度=368%; AUC ROC=053）。我们的研究结果表明，SAPA/TSSA VI在ELISA中的使用可能是一个有用的工具，研究犬自然感染克氏锥虫在流行区。
Dogs are considered the main mammal reservoir of Trypanosoma cruzi in domiciliary environments. Consequently, accurate detection of T.cruzi infection in canine populations is epidemiologically relevant. Here, we analysed the utility of the T.cruzi recombinant antigens FRA, SAPA, CP1, Ag1 and a SAPA/TSSA VI mixture, in an ELISA format. We used a positive control group of sera obtained from 38 dogs from the Chaco region in Argentina with positive homogenate-ELISA reaction, all of them also positive by xenodiagnosis and/or PCR. The negative group included 19 dogs from a nonendemic area. Sensitivity, specificity, area under the curve (AUC) of the receiver operating charactheristic (ROC) curve and Kappa index were obtained to compare the diagnostic efficiency of the tests. The SAPA/TSSA VI had the highest performance, with a sensitivity of 947% and an AUC ROC of 099 that indicates high accuracy. Among individual antigens, SAPA-ELISA yielded the highest sensitivity (868%) and AUC ROC (096), whereas FRA-ELISA was the least efficient test (sensitivity=368%; AUC ROC=053). Our results showed that the use of SAPA/TSSA VI in ELISAs could be a useful tool to study dogs naturally infected with T.cruzi in endemic areas.