A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity
A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity
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DOI：
10.1016/j.vaccine.2017.03.030
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发表时间：
2017-05-09
期刊：
影响因子：
5.5
通讯作者：
Radford, Alan D.
中科院分区：
文献类型：
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作者：
Afonso, Maria M.;Pinchbeck, Gina L.;Radford, Alan D.
背景：猫杯状病毒(FCV)是猫的一种重要病原体，经常接种疫苗。长期使用已建立的疫苗抗原增加了野外病毒可能产生抗药性的理论可能性。这项研究旨在评估FCV-F9疫苗株目前的中和能力，该疫苗株是从6个欧洲国家(英国、瑞典、荷兰、德国、法国和意大利)随机收集的当代FCV野外毒株。方法：兽医实践()随机选择6个国家。参加每次练习的猫分别从30只(英国)和40只(其他国家)猫身上采集口咽拭子。通过病毒分离确定FCV的存在，并用多变量Logistic回归分析FCV脱落的危险因素。系统发育分析用于描述FCV的种群结构。用4只免疫猫的血浆进行体外病毒中和试验，评价FCV-F9的交叉反应性。结果：FCV的总患病率为9.2%。与FCV脱落正相关的危险因素包括多猫家庭、慢性牙周炎、年龄较小、未进行绝育以及居住在某些国家。系统发育分析显示有广泛的变异性，没有全国范围的集群。尽管FCV-F9在20世纪50年代首次被分离，但它与当代的野外分离株形成了集群。FCV-F9的血浆中和效价为1：4~1：5792，其中26.5%、35.7%和50%的分离株分别被5、10和20个抗体单位中和。结论：本研究是欧洲水平上最大规模的FCV多样性和抗原交叉反应性前瞻性分析。抽样的规模和随机性质使人们相信，所使用的FCV分离株广泛代表了这些国家猫所暴露的FCV。体外中和结果表明，针对FCV-F9的抗体在抽样的欧洲国家与当代FCV分离株保持广泛的交叉反应。(C)2017年作者。爱思唯尔有限公司出版。
Background: Feline calicivirus (FCV) is an important pathogen of cats for which vaccination is regularly practised. Long-term use of established vaccine antigens raises the theoretical possibility that field viruses could become resistant. This study aimed to assess the current ability of the FCV-F9 vaccine strain to neutralise a randomly collected contemporary panel of FCV field strains collected prospectively in six European countries.Methods: Veterinary practices (64) were randomly selected from six countries (UK, Sweden, Netherlands, Germany, France and Italy). Oropharyngeal swabs were requested from 30 (UK) and 40 (other countries) cats attending each practice. Presence of FCV was determined by virus isolation, and risk factors for FCV shedding assessed by multivariable logistic regression. Phylogenetic analyses were used to describe the FCV population structure. In vitro virus neutralisation assays were performed to evaluate FCV-F9 cross reactivity using plasma from four vaccinated cats.Results: The overall prevalence of FCV was 9.2%. Risk factors positively associated with FCV shedding included multi-cat households, chronic gingivostomatitis, younger age, not being neutered, as well as residing in certain countries. Phylogenetic analysis showed extensive variability and no countrywide clusters. Despite being first isolated in the 1950s, FCV-F9 clustered with contemporary field isolates. Plasma raised to FCV-F9 neutralized 97% of tested isolates (titres 1:4 to 1:5792), with 26.5%, 35.7% and 50% of isolates being neutralized by 5, 10 and 20 antibody units respectively.Conclusions: This study represents the largest prospective analysis of FCV diversity and antigenic cross reactivity at a European level. The scale and random nature of sampling used gives confidence that the FCV isolates used are broadly representative of FCVs that cats are exposed to in these countries. The in vitro neutralisation results suggest that antibodies raised to FCV-F9 remain broadly cross-reactive to contemporary FCV isolates across the European countries sampled. (C) 2017 The Authors. Published by Elsevier Ltd.