Detection of two pathogenic marine ciliates Ancistrum haliotis and A.crassum (Ciliophora:Scuticociliatia) by fluorescence in situ hybridization
Detection of two pathogenic marine ciliates Ancistrum haliotis and A.crassum (Ciliophora:Scuticociliatia) by fluorescence in situ hybridization
复制标题
荧光原位杂交检测两种致病性海洋纤毛虫 Ancistrum haliotis 和 A.crassum (Ciliophora:Scuticociliatia)
DOI：
10.1007/s13131-020-1683-0
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发表时间：
2020
影响因子：
1.4
通讯作者：
Kuidong Xu
中科院分区：
文献类型：
--
作者：
Zifeng Zhan;Kuidong Xu
盾形纤毛虫Ancistrum haliotis和A.crassum是可能导致养殖鲍鱼和菲律宾蛤仔高死亡率的寄生虫。传统的银染鉴定方法因其与近缘物种的形态相似性和复杂的形态分析程序而受到阻碍。我们设计了两种荧光染料标记的SSU rRNA靶向寡核苷酸探针，结果表明，所建立的荧光原位杂交方法能有效地检测出两种病原菌的荧光信号，可用于快速、准确地定量分析两种病原菌对宿主贝类的感染情况。
The scuticociliatid ciliates Ancistrum haliotis and A.crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp.and the bivalve Ruditapes philippinarum.Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis.We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome,and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A.halioti and A.crassum,respectively.The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes.The method can be used for quick and accurate quantitative analysis of A.haliotis and A.crassum infections on host molluscs.