Oral microbiome in HIV-associated periodontitis.
Oral microbiome in HIV-associated periodontitis.
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DOI：
10.1097/md.0000000000005821
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发表时间：
2017-03
期刊：
影响因子：
1.6
通讯作者：
Marconi VC
中科院分区：
文献类型：
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作者：
Noguera-Julian M;Guillén Y;Peterson J;Reznik D;Harris EV;Joseph SJ;Rivera J;Kannanganat S;Amara R;Nguyen ML;Mutembo S;Paredes R;Read TD;Marconi VC
补充数字内容可在文本中找到，与 HIV 相关的牙周病 (PD) 可能是慢性炎症的根源。在这里，我们试图描述不同 PD 严重程度的 HIV+ 和 HIV- 个体的口腔微生物特征。这项横断面研究包括患有不同程度 PD 的 HIV+ 和 HIV- 患者。获取两颗牙齿、两颗脸颊和 1 份唾液样本用于微生物组分析。 Mothur/SILVADB 用于对序列进行分类。 R/Bioconductor（Vegan、PhyloSeq 和 DESeq2）用于评估整体微生物组结构差异和组间细菌属丰度差异。多色流式细胞术用于评估 CD4 和 CD8 细胞群的免疫激活。其中包括来自 50 名参与者（40 名 HIV+ 和 10 名 HIV-）的约 250 份脸颊、牙齿和唾液样本。 PD 的严重程度在临床上被分为无/轻度 (N)、中度 (M) 和重度 (S)，每个类别分别有 18 名 (36%)、16 名 (32%) 和 16 名 (32%) 参与者。在全球范围内，排序分析表明按解剖部位进行聚类（R2 = 0.25，P < 0.001）。 HIV 状态和 PD 严重程度对微生物组组成有统计学上的显着影响，但仅占变异的 2%。 HIV+ 样本中，无营养菌属、奈瑟菌属、金氏菌属和未分类的奈瑟菌科属富集，细毛菌属和月单胞菌属减少。无论采样地点和 PD 水平如何，HIV+ 参与者中的奈瑟菌属始终富集。 HIV+参与者的免疫标志物发生了改变，但并未显示出与口腔微生物群的相关性。 HIV 相关的口腔微生物组变化导致 PD 不同阶段的微妙微生物特征，这在独立的口腔解剖部位很常见。
Supplemental Digital Content is available in the text HIV-associated periodontal diseases (PD) could serve as a source of chronic inflammation. Here, we sought to characterize the oral microbial signatures of HIV+ and HIV– individuals at different levels of PD severity. This cross-sectional study included both HIV+ and HIV– patients with varying degrees of PD. Two tooth, 2 cheek, and 1 saliva samples were obtained for microbiome analysis. Mothur/SILVADB were used to classify sequences. R/Bioconductor (Vegan, PhyloSeq, and DESeq2) was employed to assess overall microbiome structure differences and differential abundance of bacterial genera between groups. Polychromatic flow cytometry was used to assess immune activation in CD4 and CD8 cell populations. Around 250 cheek, tooth, and saliva samples from 50 participants (40 HIV+ and 10 HIV–) were included. Severity of PD was classified clinically as None/Mild (N), Moderate (M), and Severe (S) with 18 (36%), 16 (32%), and 16 (32%) participants in each category, respectively. Globally, ordination analysis demonstrated clustering by anatomic site (R2 = 0.25, P < 0.001). HIV status and PD severity showed a statistically significant impact on microbiome composition but only accounted for a combined 2% of variation. HIV+ samples were enriched in genera Abiotrophia, Neisseria, Kingella, and unclassified Neisseriaceae and depleted in Leptotrichia and Selenomonas. The Neisseria genus was consistently enriched in HIV+ participants regardless of sampling site and PD level. Immune markers were altered in HIV+ participants but did not show association with the oral microbiome. HIV-associated changes in oral microbiome result in subtle microbial signatures along different stages of PD that are common in independent oral anatomic sites.