Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids
Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids
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DOI：
10.1111/j.1471-4159.2009.05925.x
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发表时间：
2009-04-01
影响因子：
4.7
通讯作者：
Luetje, Charles W.
中科院分区：
文献类型：
--
作者：
Repicky, Sarah E.;Luetje, Charles W.
哺乳动物识别和区分数千种不同气味的能力表明，气味受体的组合使用，每个受体检测多种气味，每个气味与多个受体相互作用。许多受体可以专门用于气味空间的特别重要区域的采样。在这项研究中，我们探索了四种小鼠气味受体(MOR23-1、MOR31-4、MOR32-11和MOR40-4)的分子感受范围的异同，这四种受体以前被认为是脂肪族羧酸的受体。每种受体都在非洲爪哇卵母细胞中表达，与Gα(OLF)和囊性纤维化跨膜调节因子一起表达，以允许对受体反应的电生理测试。我们发现，尽管这些受体相对无关，但它们的接受范围之间存在广泛的重叠。也就是说，这些受体对气味空间的一个相似区域进行采样。然而，每个受体的接受范围是独一无二的。因此，这些受体有助于这一小块气味空间的覆盖深度。这样一组受体具有重叠但截然不同的接受范围，可能参与在密切相关的气味化合物的复杂混合物中进行细微区分。
The ability of mammals to identify and distinguish among many thousands of different odorants suggests a combinatorial use of odorant receptors, with each receptor detecting multiple odorants and each odorant interacting with multiple receptors. Numerous receptors may be devoted to the sampling of particularly important regions of odor space. In this study, we explore the similarities and differences in the molecular receptive ranges of four mouse odorant receptors (MOR23-1, MOR31-4, MOR32-11 and MOR40-4), which have previously been identified as receptors for aliphatic carboxylic acids. Each receptor was expressed in Xenopus oocytes, along with G alpha(olf) and the cystic fibrosis transmembrane regulator to allow electrophysiological assay of receptor responses. We find that even though these receptors are relatively unrelated, there is extensive overlap among their receptive ranges. That is, these receptors sample a similar region of odor space. However, the receptive range of each receptor is unique. Thus, these receptors contribute to the depth of coverage of this small region of odor space. Such a group of receptors with overlapping, but distinct receptive ranges, may participate in making fine distinctions among complex mixtures of closely related odorant compounds.