Structural and genetic relationships of two pairs of closely related O-antigens of Escherichia coli and Salmonella enterica: E. coli O11/S. enterica O16 and E. coli O21/S. enterica O38.
Structural and genetic relationships of two pairs of closely related O-antigens of Escherichia coli and Salmonella enterica: E. coli O11/S. enterica O16 and E. coli O21/S. enterica O38.
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DOI：
10.1111/j.1574-695x.2010.00771.x
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发表时间：
2011-04
影响因子：
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通讯作者：
Yang Li;A. Perepelov;Dan Guo;S. D. Shevelev;S. Senchenkova;A. S. Shahskov;B. Liu;Lei Wang
中科院分区：
文献类型：
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作者：
Yang Li;A. Perepelov;Dan Guo;S. D. Shevelev;S. Senchenkova;A. S. Shahskov;B. Liu;Lei Wang
O-抗原是革兰氏阴性细菌外膜中脂多糖分子的一部分，对于微生物的全部功能至关重要。肠沙门氏菌和大肠杆菌是分类学上密切相关的物种。在本研究中，确定了肠沙门氏菌 O16 和 O38 以及大肠杆菌 O11 的 O 抗原结构。研究发现肠沙门氏菌 O38 和大肠杆菌 O21 具有相同的 O 抗原结构，而肠沙门氏菌 O16 和大肠杆菌 O11 具有密切相关的结构，区别仅在于前者存在侧向葡萄糖残基和甘露糖残基的 O-乙酰化。将肠沙门氏菌O16和O38以及大肠杆菌O11的O抗原基因簇与从GenBank检索到的大肠杆菌O21的O抗原基因簇一起进行测序和分析。每个肠沙门氏菌/E。发现大肠杆菌对包含以相同方式组织的同一组基因，并且总体 DNA 同一性为 56-78%。这些数据表明，所研究的每对 O 抗原基因簇都起源于共同的祖先。因此，很明显，在过去，肠沙门氏菌和大肠杆菌的O-抗原之间的相关性程度被低估了。
The O-antigen is a part of the lipopolysaccharide molecule present in the outer membrane of Gram-negative bacteria, and is essential for the full function of the microorganisms. Salmonella enterica and Escherichia coli are taxonomically closely related species. In this study, the O-antigen structures of S. enterica O16 and O38 and E. coli O11 were determined. Salmonella enterica O38 and E. coli O21 were found to have identical O-antigen structures, whereas S. enterica O16 and E. coli O11 had closely related structures, differing only in the presence of a lateral glucose residue and O-acetylation of a mannose residue in the former. The O-antigen gene clusters of S. enterica O16 and O38 and E. coli O11 were sequenced and analyzed together with that of E. coli O21 retrieved from the GenBank. Each S. enterica/E. coli pair was found to contain the same set of genes organized in the same manner and to share 56-78% overall DNA identity. These data suggest that the O-antigen gene clusters of each pair studied originated from a common ancestor. Thus, it has become evident that in the past, the degree of relatedness between the O-antigens of S. enterica and E. coli was underestimated.