Fluorescence correlation spectroscopy in living cells: a practical approach.
Fluorescence correlation spectroscopy in living cells: a practical approach.
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DOI：
10.1002/0471143030.cb0424s45
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Altan-Bonnet, Gregoire
中科院分区：
文献类型：
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作者：
Altan-Bonnet, Nihal;Altan-Bonnet, Gregoire
荧光相关光谱（FCS）是一种用于监测分子动力学的单分子荧光技术。它可应用于表达荧光标记蛋白质和脂质的活细胞，以确定细胞中扩散荧光分子的扩散时间尺度和总数。本单元介绍了使用商用光谱/显微镜系统 Zeiss Confocor 3 执行 FCS 的实用分步方法、如何设置活细胞 FCS 实验、获取可靠数据以及最终如何分析所获取的数据。
Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit.