5-Aminolevulinic acid alleviates the salinity-induced changes in Brassica napus as revealed by the ultrastructural study of chloroplast
5-Aminolevulinic acid alleviates the salinity-induced changes in Brassica napus as revealed by the ultrastructural study of chloroplast
复制标题
叶绿体超微结构研究表明，5-氨基乙酰丙酸可减轻甘蓝型油菜盐度引起的变化
DOI：
10.1016/j.plaphy.2012.05.018
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发表时间：
2012-08-01
影响因子：
6.5
通讯作者：
Zhou, Weijun
中科院分区：
文献类型：
--
作者：
Naeem, Muhammad S.;Warusawitharana, Hasitha;Zhou, Weijun
5-氨基乙酰丙酸（ALA）是一种重要的植物生长调节剂，是由5-碳脂肪族氨基酸衍生而成。以油菜（Brassica napus）为试材，研究了盐胁迫和ALA对油菜生长、叶片色素组成、叶片和根Na+/K+比值以及叶肉细胞叶绿体超微结构的影响。植物水培处理与三个不同的盐度水平（0，100，200毫米）和叶面应用ALA（30毫克升（-1））同时。处理10 d后，高盐胁迫显著降低了植株的生物量和株高。而ALA处理则恢复了盐胁迫下的生物量和株高。在B地上部分观察到Na+吸收的浓度依赖性增加。油菜属植物。另一方面，ALA减少Na+吸收，导致Na+/K+比值显著降低。Na+的积累增加了氧化应激，这是明显的电子显微镜图像，突出了几个变化，细胞的形状和大小，叶绿体肿胀，plastogloubli的数量增加，减少淀粉颗粒和扩张的类囊体。叶片施用ALA改善了能量供应和投资机制（更高的叶绿素和类胡萝卜素含量，提高光合效率），减少了氧化胁迫，这一点由具有更完整的类囊体的规则形状的叶绿体所证明。根据这些结果，我们可以认为ALA是一种很有前途的植物生长调节剂，可以提高植物在盐胁迫下的存活率。(C)2012年Elsevier Masson SAS。All rights reserved.
5-Aminolevulinic acid (ALA) is an important plant growth regulator which is derived from 5-carbon aliphatic amino acid. The present study investigates the interaction of increasing NaCl-salinity and ALA on plant growth, leaf pigment composition, leaf and root Na+/K+ ratio and chloroplast ultrastructure in mesophyll cells of oilseed rape (Brassica napus) leaves. The plants were treated hydroponically with three different salinity levels (0, 100, 200 mM) and foliar application of ALA (30 mg l(-1)) simultaneously. Ten days after treatment, higher NaCl-salinity significantly reduced the plant biomass and height. However, ALA application restored the plant biomass and plant height under saline conditions. A concentration-dependent increase in Na+ uptake was observed in the aerial parts of B. napus plants. On the other hand, ALA reduced Na+ uptake, leading to a significant decrease in Na+/K+ ratio. Accumulation of Na+ augmented the oxidative stress, which was evident by electron microscopic images, highlighting several changes in cell shape and size, chloroplast swelling, increased number of plastogloubli, reduced starch granules and dilations of the thylakoids. Foliar application of ALA improved the energy supply and investment in mechanisms (higher chlorophyll and carotenoid contents, enhanced photosynthetic efficiency), reduced the oxidative stress as evident by the regular shaped chloroplasts with more intact thylakoids. On the basis of these results we can suggest that ALA is a promising plant growth regulator which can improve plant survival under salinity. (C) 2012 Elsevier Masson SAS. All rights reserved.