A FIBROBLAST-ASSOCIATED ANTIGEN - CHARACTERIZATION IN FIBROBLASTS AND IMMUNOREACTIVITY IN SMOOTH-MUSCLE DIFFERENTIATED STROMAL CELLS
A FIBROBLAST-ASSOCIATED ANTIGEN - CHARACTERIZATION IN FIBROBLASTS AND IMMUNOREACTIVITY IN SMOOTH-MUSCLE DIFFERENTIATED STROMAL CELLS
复制标题
DOI：
10.1177/40.4.1552184
复制
发表时间：
1992-04-01
影响因子：
3.2
通讯作者：
PETERSEN, OW
中科院分区：
文献类型：
--
作者：
RONNOVJESSEN, L;CELIS, JE;PETERSEN, OW
具有平滑肌分化的成纤维细胞通常来源于人乳腺组织。 免疫荧光细胞化学的成纤维细胞相关抗原识别的单克隆抗体（MAb），1B10，进行了分析，以区分平滑肌分化成纤维细胞血管平滑肌细胞。 在细胞表面和成纤维细胞的组织蛋白酶D阳性和吖啶橙聚集囊泡隔室中检测到抗原。 在超微结构上，抗原显示在包被的小凹和内体和溶酶体结构中。 1B10识别出在表观M（r）38，000、45，000和80，000处迁移的三个主要品牌，此外还有许多在M（r）45，000和97，000之间的次要条带，包括M（r）52，000。 M（r）45，000和38，000与细胞膜结合，M（r）52，000和M（r）38，000与溶酶体结合。 1B10免疫反应性对血管平滑肌细胞背景下的成纤维细胞和平滑肌分化成纤维细胞具有特异性。
Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent M(r) of 38,000, 45,000, and 80,000, in addition to many minor bands between M(r) 45,000 and 97,000, including M(r) 52,000. The M(r) 45,000 and 38,000 were associated with the cell membrane and M(r) 52,000 as well as M(r) 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.