Asymmetric recruitment and actin-dependent cortical flows drive the neuroblast polarity cycle
Asymmetric recruitment and actin-dependent cortical flows drive the neuroblast polarity cycle
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DOI：
10.7554/elife.45815
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发表时间：
2019-05-08
期刊：
影响因子：
7.7
通讯作者：
Prehoda, Kenneth E.
中科院分区：
文献类型：
--
作者：
Oon, Chet Huan;Prehoda, Kenneth E.
在果蝇成神经细胞的不对称分裂过程中，Par极性复合物在细胞质和顶端皮质域之间循环，该顶端皮质域将分化因子限制在基底皮质。我们使用全细胞体积的快速成像来揭示成神经细胞极性状态之间转换的动态步骤。最初，Par蛋白aPKC和Bazooka在顶端皮质形成离散的病灶。病灶生长成斑块，这些斑块一起构成不连续的、无组织的结构。协调的皮质流开始接近中期，并依赖于肌动蛋白细胞骨架迅速转变成一个高度组织化的顶帽补丁。在分裂后期开始时，随着皮质流反向朝向出现的卵裂沟，帽解体。分裂后，皮质斑块消散到细胞质中，使成神经细胞极性循环再次开始。我们的工作表明，神经母细胞如何使用不对称的招聘和皮质流动态地在不对称的分裂周期。
During the asymmetric divisions of Drosophila neuroblasts, the Par polarity complex cycles between the cytoplasm and an apical cortical domain that restricts differentiation factors to the basal cortex. We used rapid imaging of the full cell volume to uncover the dynamic steps that underlie transitions between neuroblast polarity states. Initially, the Par proteins aPKC and Bazooka form discrete foci at the apical cortex. Foci grow into patches that together comprise a discontinuous, unorganized structure. Coordinated cortical flows that begin near metaphase and are dependent on the actin cytoskeleton rapidly transform the patches into a highly organized apical cap. At anaphase onset, the cap disassembles as the cortical flow reverses direction toward the emerging cleavage furrow. Following division, cortical patches dissipate into the cytoplasm allowing the neuroblast polarity cycle to begin again. Our work demonstrates how neuroblasts use asymmetric recruitment and cortical flows to dynamically polarize during asymmetric division cycles.