Chloroplast biogenesis. Detection of monovinyl protochlorophyll(ide) b in plants.
Chloroplast biogenesis. Detection of monovinyl protochlorophyll(ide) b in plants.
复制标题
叶绿体生物发生。
DOI：
10.1016/s0021-9258(19)47352-3
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
C. Rebeiz
中科院分区：
文献类型：
--
作者：
V. Shedbalkar;I. Ioannides;C. Rebeiz
描述了原叶绿素B和原叶绿素B植基酯在绿色植物中的存在。通过质子核磁共振、快原子轰击质谱和化学衍生化结合电子光谱分析确定了原叶绿素B植酯的化学结构。原叶绿素（ide）B b的大环与常规原叶绿素（ide）的大环相同，除了在大环的3位存在甲酰基而不是甲基。它们与叶绿素（ide）B的不同之处在于在大环的7位和8位存在氧化双键。原叶绿素（ide）B的俗名是为了将这两种四吡咯与传统的原叶绿素（ide）区分开来，而传统的原叶绿素（ide）又被称为原叶绿素（ide）a。原叶绿素（ide）B广泛分布于绿色植物中。报道了它在80%丙酮和乙醚中的摩尔消光系数。这一发现的叶绿素a和B生物合成途径的异质性的影响进行了讨论。
The occurrence of protochlorophyllide b and protochlorophyllide b phytyl ester in green plants is described. The chemical structure of protochlorophyllide b phytyl ester was established by proton nuclear magnetic resonance, fast atom bombardment mass spectroscopic analysis, and chemical derivatization coupled to electronic spectroscopic analysis. The macrocycles of protochlorophyll(ide) b are identical to those of conventional protochlorophyll(ide) except for the presence of a formyl group instead of a methyl group at position 3 of the macrocycles. They differ from chlorophyll(ide) b by the presence of an oxidized double bond at positions 7 and 8 of the macrocycles. The trivial name protochlorophyll(ide) b is proposed to differentiate these two tetrapyrroles from conventional protochlorophyll(ide), which in turn will be referred to as protochlorophyll(ide) a. Protochlorophyll(ide) b appears to be widely distributed in green plants. Its molar extinction coefficients in 80% acetone and diethyl ether are reported. The impact of this discovery on the heterogeneity of the chlorophyll a and b biosynthetic pathways is discussed.