Disulfiram-ethanol reaction in the rat. 1. Blood alcohol, acetaldehyde, and liver aldehyde dehydrogenase relationships.
Disulfiram-ethanol reaction in the rat. 1. Blood alcohol, acetaldehyde, and liver aldehyde dehydrogenase relationships.
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DOI：
10.1111/j.1530-0277.1986.tb05612.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Jensen;M. D. Faiman
中科院分区：
文献类型：
--
作者：
J. Jensen;M. D. Faiman
进行研究以确定大鼠中的双硫仑-乙醇反应（DER）是否与血液乙醛、乙醇和肝醛脱氢酶（ALDH）抑制相关。低体温和低血压均被用作DER的指标。向雌性Sprague-Dawley大鼠给予双硫仑（DSF）（100 mg/kg，i. p.）并测定低和高肝ALDH。DSF预处理对低Km ALDH的抑制作用依赖于DSF预处理时间，在DSF后6、8和12小时观察到显著抑制。在只接受乙醇的大鼠中，在120分钟内达到最大血液乙醇。血液乙醛几乎检测不到。未观察到大鼠核心温度的变化。在用DSF（100 mg/kg，i. p.）乙醇激发前8小时（1 g/kg，i. p.），发现血液乙醛显著增加，并且在整个温度和血压监测期间保持升高。血乙醇在90 min内达到最大值，然后下降。最大的低温和低血压发生后120分钟乙醇。多巴胺受体阻断剂匹莫齐特（0.5mg/kg，i. p.）乙醇刺激前60 min，可减轻低体温和低血压。匹莫齐特是有效的，无论是60分钟前乙醇或30分钟后乙醇。DER期间体温过低和低血压的发生和持续时间似乎与血液乙醇的上升和下降有关，但与血液乙醛无关。（250字处删节）
Studies were carried out to determine whether the disulfiram-ethanol reaction (DER) in the rat could be correlated with blood acetaldehyde, ethanol, and liver aldehyde dehydrogenase (ALDH) inhibition. Both hypothermia and hypotension were used as indices of the DER. Female Sprague-Dawley rats were given disulfiram (DSF) (100 mg/kg, i.p.) and low and high liver ALDH determined. No effect on high Km ALDH was found. Inhibition of low Km ALDH was dependent on DSF pretreatment time, with significant inhibition observed at 6, 8, and 12 hr following DSF. In rats receiving ethanol only, maximal blood ethanol was reached within 120 min. Blood acetaldehyde was almost undetectable. No change in rat core temperature was observed. In rats pretreated with DSF (100 mg/kg, i.p.) 8 hr before ethanol challenge (1 g/kg, i.p.), a marked increase in blood acetaldehyde was found and remained elevated throughout the temperature and blood pressure monitoring period. Blood ethanol reached a maximum within 90 min and then declined. Maximal hypothermia and hypotension occurred 120 min after ethanol. The administration of the dopamine receptor blocker pimozide (0.5 mg/kg, i.p.) 60 min before ethanol challenge, attenuated the hypothermia and hypotension. Pimozide was effective when given either 60 min before ethanol or 30 min after ethanol. The onset and duration of hypothermia and hypotension during the DER appears to follow the rise and fall of blood ethanol but not blood acetaldehyde.(ABSTRACT TRUNCATED AT 250 WORDS)