Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males
Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males
复制标题
DOI：
10.1091/mbc.e05-02-0096
复制
发表时间：
2005-09-01
影响因子：
3.3
通讯作者：
Baylis, HA
中科院分区：
文献类型：
--
作者：
Gower, NJD;Walker, DS;Baylis, HA
复杂的行为需要神经系统和非神经元目标的协调行动。秀丽隐杆线虫的雄性交配由一系列明确的行为步骤组成，这些步骤导致成功怀孕所需的生理结果。我们证明，交配过程中的几个点需要肌醇 1,4,5-三磷酸 (IP3) 介导的信号传导。由于转动行为（外阴定位期间）、骨针插入和精子转移方面的缺陷，IP3 受体 (itr-1) 功能的破坏会导致男性生育能力的急剧丧失。为了阐明相关的信号传导途径，我们敲除了编码磷脂酶 C (PLC) 家族成员的 6 个线虫基因。 egl-8 编码 PLC-beta，在针状体插入和精子转移中发挥作用。 itr-1和egl-8在男性生殖系统中广泛表达。 itr-1 功能获得性突变可挽救由egl-8 RNA 干扰引起的不孕症，表明egl-8 和itr-1 共同作为控制精子转移的信号事件的核心组成部分发挥作用。
Complex behavior requires the coordinated action of the nervous system and nonneuronal targets. Male mating in Caenorhabditis elegans consists of a series of defined behavioral steps that lead to the physiological outcomes required for successful impregnation. We demonstrate that signaling mediated by inositol 1,4,5-trisphosphate (IP3) is required at several points during mating. Disruption of IP3 receptor (itr-1) function results in dramatic loss of male fertility, due to defects in turning behavior (during vulva location), spicule insertion and sperm transfer. To elucidate the signaling pathways responsible, we knocked down the six C. elegans genes encoding phospholipase C (PLC) family members. egl-8, which encodes PLC-beta, functions in spicule insertion and sperm transfer. itr-1 and egl-8 are widely expressed in the male reproductive system. An itr-1 gain-of-function mutation rescues infertility caused by egl-8 RNA interference, indicating that egl-8 and itr-1 function together as central components of the signaling events controlling sperm transfer.