Molecular characterization of
Molecular characterization of
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DOI：
10.1007/s10658-023-02696-x
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发表时间：
2023-05
影响因子：
1.8
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中科院分区：
文献类型：
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几种短梗霉属对2022年冬季从野外环境中分离的菌株进行了内转录间隔区（ITS）、翻译延伸因子EF-1α基因（EF 1）和部分延伸酶基因（ELO）的序列分析。EF 1和Ep位点的变异性高于ITS。除1株（UC 14）外，所有菌株均鉴定为阿萨A。普鲁兰多糖。为了评估所表征的菌株作为采后贮藏期间发生的疾病的生物控制剂（BCA）的有效性，通过体外和体内测定来评估所选择的菌株。平均而言，Moniliniaspp.非挥发性代谢物的菌落生长比挥发性（VOC）的菌落生长更显著。菌株UC 14对桃褐腐病菌VOCs的菌丝生长抑制作用最强（66%）。室内试验结果表明，各菌株对冷藏期间褐腐病均有较好的防治效果，对褐腐菌的生长抑制作用显著。其中VB 23对褐腐病的防治效果最好，褐腐病发病率分别提高80%、60%、100%，严重度分别提高79.5%、72.7%、100%; fructicola和M. polystroma，分别。
SeveralAureobasidiumspp. strains isolated from wild environments during winter 2022 were characterized by sequence analysis of the internal transcribed spacer (ITS), the translation elongation factor EF-1α gene (EF1), and part of the elongase gene (ELO). The variability in the EF1 and ELOlociare higher than in the ITS. All strains but one (UC14), were identified asA. pullulans. To assess the effectiveness of the characterized strains as biocontrol agents (BCAs) of diseases occurring during postharvest storage, a selection of the strains was evaluated byin vitroandin vivoassays. On average, the reduction ofMoniliniaspp. colony growth was more marked for non-volatile metabolites than for volatile (VOCs). Strain UC14 provided the strongest mycelial growth reduction ofMonilinia fructicolaby VOCs (66%). According to thein vivoresults,all strains were effective in controlling brown rot during cold storage and remarkably in restricting the growth ofMonilinia polystroma. In particular, VB23 was the most effective in controlling brown rot incidence, by 80%, 60%, 100%, and severity, by 79.5%, 72.7% and 100%, forMonilinia laxa, M. fructicola,andM. polystroma,respectively.