Fragments of ATP synthase mediate plant perception of insect attack
Fragments of ATP synthase mediate plant perception of insect attack
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DOI：
10.1073/pnas.0602328103
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发表时间：
2006-06-06
影响因子：
11.1
通讯作者：
Teal, PEA
中科院分区：
文献类型：
--
作者：
Schmelz, EA;Carroll, MJ;Teal, PEA
植物可以感知各种生物攻击者，并以有针对性的诱导防御做出反应。植物非自我识别的特异性直接通过感知害虫衍生的激发子或间接通过不适当蛋白水解的宿主靶标的抗性蛋白识别而发生。间接的植物感知可以发生在与病原体的相互作用期间，但介导昆虫食草动物检测的类似事件的证据仍然难以捉摸。在这里，我们报告的草食性豇豆（豇豆）植物秋粘虫（草地贪夜蛾）幼虫攻击的间接感知。我们分离并鉴定了一个二硫键桥连肽（（+）ICD 1 NGVCVDA（-）），命名为inceptin。frugiperda幼虫的口腔分泌物，促进豇豆乙烯生产在1 fmol叶（-1），并触发增加防御相关的植物激素水杨酸和茉莉酸。起始素是叶绿体ATP合成酶γ亚基调节区的蛋白水解片段，其通过诱导挥发性、苯丙烷类和蛋白酶抑制剂防御来介导植物对植食性的感知。只有S。frugiperda幼虫，以前摄取叶绿体ATP合酶γ-亚基蛋白和产生inceptin显着诱导豇豆防御后，草食性。作为一种古老的和必需的植物酶的消化片段，inceptin作为一种有效的间接信号启动植物对昆虫攻击的特异性反应。
Plants can perceive a wide range of biotic attackers and respond with targeted induced defenses. Specificity in plant non-self-recognition occurs either directly by perception of pest-derived elicitors or indirectly through resistance protein recognition of host targets that are inappropriately proteolyzed. indirect plant perception can occur during interactions with pathogens, yet evidence for analogous events mediating the detection of insect herbivores remains elusive. Here we report indirect perception of herbivory in cowpea (Vigna unguiculata) plants attacked by fall armyworm (Spodoptera frugiperda) larvae. We isolated and identified a disulfide-bridged peptide ((+)ICDlNGVCVDA(-)), termed inceptin, from S. frugiperda larval oral secretions that promotes cowpea ethylene production at 1 fmol leaf(-1) and triggers increases in the defense-related phytolhormones salicylic acid and jasmonic acid. Inceptins are proteolytic fragments of chloroplastic ATP synthase gamma-subunit regulatory regions that mediate plant perception of herbivory through the induction of volatile, phenylpropanoid, and protease inhibitor defenses. Only S. frugiperda larvae that previously ingested chloroplastic ATP synthase gamma-subunit proteins and produced inceptins significantly induced cowpea defenses after herbivory. Digestive fragments of an ancient and essential plant enzyme, inceptin functions as a potent indirect signal initiating specific plant responses to insect attack.