Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial
Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial
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DOI：
10.1088/1748-605x/aa8fa1
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发表时间：
2018-03-01
影响因子：
4
通讯作者：
Noh, Insup
中科院分区：
文献类型：
--
作者：
Das, Dipankar;Zhang, Shengmin;Noh, Insup
以海藻酸盐(Alg)、α-磷酸三钙(α-TcP)微粒和氯化钙为生物材料，通过离子交联法制备了生物相容性杂化薄膜。Alg-α-Tcp薄膜的制备采用了“熨平法”(类似于混凝土涂饰工艺)。在这种方法中，Alg/α-Tcp共混物是用超声波制备的，然后放在玻璃片上。在此之后，通过沿混合物表面滑动另一张幻灯片来切断混合物的多余体积，以获得适当的等级和平整度。通过改变膜的组成来控制膜(Alg-α-TCP)的机械强度和柔韧性。通过衰减全反射傅里叶变换红外光谱(ATR-FTIR)、核磁共振(C-13)、X-射线衍射谱和热重分析证实了该交联现象。ATR-FTIR和C-13核磁共振分析结果表明，藻酸盐中的羧基与钙离子发生了离子交联，而α-磷酸三钙粒子则以物理作用的形式存在于网络中。微观疲劳测试结果表明，Alg-α-TCP杂化薄膜具有高的抗拉强度(高达257兆帕)和柔韧性(高达13%的伸长率)。扫描电子显微镜分析表明，α-磷酸三钙颗粒均匀分布在Alg-α-磷酸三钙薄膜的表面，而横截面图像证实了交联型网络中α-磷酸三钙的存在。热重分析结果表明，由于海藻酸盐与α-磷酸三钙之间的离子交联和界面相互作用，杂化膜的热稳定性得到了提高。α-磷酸三钙颗粒的加入降低了杂化薄膜的溶胀比。体外骨细胞(MC3T3)培养和细胞毒性试验表明，杂化膜具有良好的生物相容性。杂化膜在37℃、pH=7和7.4的条件下可控制释放牛血清白蛋白和二羟甲基丙酰甘氨酸，具有良好的机械强度和柔韧性，可作为界面膜应用于组织工程。
A biocompatible hybrid film has been fabricated using alginate (Alg), alpha-tricalcium phosphate (alpha-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial. The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-alpha-TCP film. For this method, the Alg/alpha-TCP blend has been prepared using an ultra-sonicator and then put on a glass slide. After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness. The mechanical strength and flexibility of the film (Alg-alpha-TCP) has been controlled by changing its compositions. The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), C-13 nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses. The ATR-FTIR and C-13 NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the alpha-TCP particles reside in the network by physical interaction. The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-alpha-TCP hybrid films. The SEM analysis suggests the alpha-TCP particles are homogeneously distributed on the surface of Alg-alpha-TCP films, whereas cross-sectional images confirmed the presence of alpha-TCP in the cross-linked network. TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and alpha-TCP. The incorporation of alpha-TCP particles diminished the swelling ratio of the hybrid film. The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible. The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH7 and 7.4, and 37 degrees C. Overall, the biocompatible Alg-alpha-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.