Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs)
Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs)
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DOI：
10.1016/j.theriogenology.2005.09.016
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发表时间：
2006-06-01
期刊：
影响因子：
2.8
通讯作者：
Taft, Rob A.
中科院分区：
文献类型：
--
作者：
Byers, Shannon L.;Payson, Suzan J.;Taft, Rob A.
超排卵、体外受精、胚胎冷冻保存和胚胎移植是广泛应用于实验小鼠的辅助生殖技术。近亲繁殖的小鼠具有内在的遗传差异，导致它们对这些技术的反应不同。了解常见的近交系在用于抗逆转录病毒治疗时的表现将确保最有效地利用小鼠、时间和资源。本研究研究了129S1/ SvImJ、A/J、BALB/cJ、BALB/cByJ、C3H/HeJ、C57BL/6J、DBA/2J、FVB/NJ、NOD/LtJ和SJL/J 10个近交系在胚胎移植后的超排卵、体外受精和产仔能力。使用eCG （5.0 IU）和hCG （5.0 IU）对三周龄雌性小鼠进行超排卵。所得到的卵母细胞与同一菌株的精子在人输卵管液培养基中体外受精。第二天，将两细胞胚胎移植到假怀孕的雌性受体中或冷冻保存。冷冻保存的胚胎随后解冻并转移到假怀孕的雌性受体。不同菌株对超排卵、受精和胚胎移植后的活产数的反应存在差异，证实了遗传变异对抗逆转录病毒的影响。不同菌株对超排卵治疗的反应不同，从每只雌性5 +/- 1 （A/J）到40 +/- 3 （129S1/SvImJ）正常卵母细胞不等。卵母细胞受精率平均为24% (129S1/SvImJ) ~ 93% （DBA/2J和A/J）。新鲜胚胎的双细胞胚胎移植到雌性受体体内并发育成活幼崽的平均比例为5% (A/J) ~ 53% (C57BL/6J)，解冻胚胎的平均比例为18% (BALB/cByJ) ~ 45% （129S1/SvImJ）。(c) 2005爱思唯尔公司版权所有。
Superovulation, in vitro fertilization, embryo cryopreservation, and embryo transfer are assisted reproductive technologies (ARTs) widely used in laboratory mice. Inbred strains of mice have inherent genetic differences that cause them to respond differently to these technologies. Knowing how common inbred strains will perform when used for ARTs will ensure the most efficient use of mice, time, and resources. In this study, we characterized the ability of 10 inbred strains: 129S1/ SvImJ, A/J, BALB/cJ, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, FVB/NJ, NOD/LtJ, and SJL/J to superovulate, fertilize in vitro, and produce live pups subsequent to embryo transfer. Three-week-old female mice were superovulated using eCG (5.0 IU) and hCG (5.0 IU). The resulting oocytes were fertilized in vitro in human tubal fluid medium with spermatozoa of the same strain. The following day, two-cell embryos were either transferred into pseudopregnant recipient females or cryopreserved. The cryopreserved embryos were later thawed and transferred into pseudopregnant recipient females. Differences in response to superovulation, fertilization, and number of live born produced after embryo transfer were observed between strains, substantiating the influence of genetic variability on ARTs. The response to the superovulation treatment varied among strains and ranged from 5 +/- 1 (A/J) to 40 +/- 3 (129S1/SvImJ) normal oocytes per female. The average proportion of oocytes that fertilized ranged among strains from 24% (129S1/SvImJ) to 93% (DBA/2J and A/J). The average proportion of two-cell embryos that were transferred into recipient females and subsequently developed into live pups varied from 5% (A/J) to 53% (C57BL/6J) for fresh embryos and from 18% (BALB/cByJ) to 45% (129S1/SvImJ) for thawed embryos. (c) 2005 Elsevier Inc. All rights reserved.