Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice.
Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice.
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DOI：
10.55782/ane-2014-2010
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发表时间：
2014-12
影响因子：
1.4
通讯作者：
C. Mannoji;M. Koda;Koshiro Kamiya;M. Dezawa;Masayuki Hashimoto;T. Furuya;A. Okawa;Kazuhisa Takahashi;M. Yamazaki
中科院分区：
文献类型：
--
作者：
C. Mannoji;M. Koda;Koshiro Kamiya;M. Dezawa;Masayuki Hashimoto;T. Furuya;A. Okawa;Kazuhisa Takahashi;M. Yamazaki
骨髓基质细胞（BMSCs）移植治疗脊髓损伤（SCI）已被证明可以改善功能结果。骨髓间充质干细胞来源广泛，从伦理学和法律的角度来看，骨髓间充质干细胞在临床上应用于脊髓损伤的问题较少。最近，我们从人骨髓基质细胞（人骨髓基质细胞衍生的神经再生细胞：hBMSC-NR）中产生了具有神经干细胞和/或祖细胞特性和神经再生支持能力的细胞。本研究的目的是阐明hBMSC-NRs移植到严重联合免疫缺陷（NOD/SCID）小鼠脊髓损伤的有效性。进行PC-12细胞的神经突生长测定。在T9水平挫伤SCI后一周，将hBMSCs或hBMSC-NRs移植到脊髓中。移植后进行功能和组织学检查。hBMSC-NRs条件培养液能显著促进PC-12细胞突起生长。脊髓损伤后8周，移植的hBMSC-NRs在损伤脊髓内存活。免疫组化结果显示，移植组脊髓损伤中心区和尾侧段脊髓损伤区的脊髓损伤后角肌纤维密度明显高于对照组。hBMSC-NRs组大鼠后肢功能恢复明显优于对照组。总之，hBMSC-NRs可以成为人类SCI细胞移植治疗的现实候选者之一。
Transplantation of bone marrow stromal cells (BMSCs) for spinal cord injury (SCI) has been shown to improve functional outcome. BMSCs can be easily obtained from bone marrow aspirate and have fewer problems in the clinical application for human SCI from the ethical and legal points of view. Recently, we produced cells with neural stem and/or progenitor cell property and neural regeneration supporting capacity from human bone marrow stromal cells (human bone marrow stromal cell-derived neuroregenerative cells: hBMSC-NRs). The aim of the present study was to clarify the effectiveness of transplantation of hBMSC-NRs to injured spinal cord of severe combined immunodeficiency (NOD/SCID) mice. Neurite outgrowth assay of PC-12 cells was performed. One week after a T9-level contusion SCI, hBMSCs or hBMSC-NRs were transplanted into the spinal cord. After the transplantation, functional and histological examinations were performed. Conditioned media of hBMSC-NRs significantly promoted the neurite outgrowth of PC-12 cells in vitro. Transplanted hBMSC-NRs survived in the injured spinal cord 8 weeks after SCI. Immunohistochemistry revealed that the density of serotonin-positive fibers of the transplanted group was significantly higher than that of the control group at the epicenter and caudal segment to the injured site. The recovery of hind limb function of the hBMSC-NRs group was significantly better than that of the control group. In conclusion, hBMSC-NRs can be one of the realistic candidates for cell transplantation therapy for human SCI.