Iron regulated genes of Salmonella enterica serovar Typhimurium in response to norepinephrine and the requirement of fepDGC for norepinephrine-enhanced growth
Iron regulated genes of Salmonella enterica serovar Typhimurium in response to norepinephrine and the requirement of fepDGC for norepinephrine-enhanced growth
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DOI：
10.1016/j.micinf.2008.04.011
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发表时间：
2008-06-01
影响因子：
5.8
通讯作者：
Lay, Donald C., Jr.
中科院分区：
文献类型：
--
作者：
Bearson, Bradley L.;Bearson, Shawn M. D.;Lay, Donald C., Jr.
儿茶酚胺在体内可通过两种机制刺激肠道细菌，包括食源性致病菌沙门氏菌、鼠伤寒沙门氏菌和铁的供应者。为了鉴定鼠伤寒沙门氏菌对去甲肾上腺素的应答基因，进行了转座子突变和DNA微阵列分析。以下基因的插入突变减少了去甲肾上腺素促进的生长：degs、ente、entf、fes、gpmA、hfq、STM3846。DNA芯片和实时RT-PCR分析显示，在去甲肾上腺素暴露过程中，与铁的获取和利用有关的几个基因的表达减少，这表明血清SAPI最低限度的铁限制条件和去甲肾上腺素的铁载体样活性。与野生型亲本菌株不同的是，去甲肾上腺素促进了Fepa Iron ch-A突变体和FePc突变体的生长，它们分别在肠球蛋白的外膜和内膜转运体上存在缺失突变。然而，FePc和Fepa铁ch-A突变体的生长可以被另一种铁载体铁氧胺E拯救，进一步验证了去甲肾上腺素通过儿茶酚特异的铁运输系统向生物体供应铁的作用。与以前使用小动物模型的报告相反，鼠伤寒沙门氏菌的Fepa Iron CIRA突变株和FePc突变株一样，在猪的胃肠道中定植。爱思唯尔·马森公司出版。
Catecholamines may stimulate enteric bacteria including the foodborne pathogen Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) by two mechanisms in vivo: as a quorum sensing signal and a supplier of iron. To identify genes of Salmonella Typhimurium that respond to norepinephrine, transposon mutagenesis and DNA microarray analysis were performed. Insertional mutations in the following genes decreased norepinephrine-enhanced growth: degS, entE, entF, fes, gpmA, hfq, STM3846. DNA microarray and real-time RT-PCR analyses revealed a decrease in the expression of several genes involved in iron acquisition and utilization during norepinephrine exposure, signifying the iron-limiting conditions of serum-SAPI minimal medium and the siderophore-like activity of norepinephrine. Unlike the wild-type parent strain, growth of neither a fepA iroN ch-A mutant nor a fepC mutant, harboring deletional mutations in the outer and inner membrane transporters of enterochelin, respectively, was enhanced by norepinephrine. However, growth of the fepC and the fepA iroN ch-A mutants could be rescued by an alternative siderophore, ferrioxamine E, further validating the role of norepinephrine in supplying the organism with iron via the catecholate-specific iron transport system. Contrary to previous reports using small animal models, the fepA iroN cirA mutant of Salmonella Typhimurium colonized the swine gastrointestinal tract, as did the fepC mutant. Published by Elsevier Masson SAS.