Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis
Soy moderately improves microstructural properties without affecting bone mass in an ovariectomized rat model of osteoporosis
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DOI：
10.1016/j.bone.2005.10.024
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发表时间：
2006-05-01
期刊：
影响因子：
4.1
通讯作者：
Arjmandi, Bahram H.
中科院分区：
文献类型：
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作者：
Devareddy, Latha;Khalil, Dania A.;Arjmandi, Bahram H.
据报道，大豆蛋白可以防止女性和骨质疏松症大鼠模型的骨质流失。然而，大豆异黄酮对骨小梁微结构特性的作用还有待进一步研究。在本研究中，我们研究了大豆蛋白与不同剂量的大豆酮是否逆转了卵巢切除（Ovx）骨质疏松大鼠模型的骨矿物质密度（BMD）、骨矿物质含量（BMC）和骨小梁显微结构的损失。将78只9月龄雌性Sprague-Dawley大鼠进行假手术（Sham; 1组）或Ovx（5组），并喂食半纯化的酪蛋白基饮食。90天后，使用双能X线吸收测定法确认骨丢失的发生。此后，将大鼠分配至以下处理：假手术、Ovx（对照）、Ovx + 17 β-雌二醇（E-2; 10 μ g/kg体重）。每周两次）、Ovx +去除了异黄酮的大豆蛋白（大豆-; 0.06mg异黄酮/g蛋白）、Ovx +具有正常异黄酮含量的大豆蛋白（大豆; 3.55mg异黄酮/g蛋白）和Ovx +富含异黄酮的大豆蛋白（大豆+; 7.10mg异黄酮/g蛋白）。治疗125天后，将大鼠安乐死，并收集胫骨和腰椎骨，用于使用X射线微计算机断层扫描评估BMD、BMC和小梁微结构特性。没有一种治疗对腰椎的BMD或微结构特性有影响。然而，大豆治疗显著增加胫骨BMC和BMD的10%和4.5%，与卵巢对照组相比，但增加的BMD不足以达到假手术对照组的BMD水平。大豆（+）饮食积极影响胫骨结构特性，包括骨小梁厚度，分离和数量。总之，我们的研究结果表明，大豆蛋白不能恢复骨质疏松大鼠的骨丢失;然而，可能需要更高剂量的大豆酮来逆转胫骨微观结构特性的丧失。(c)2005年爱思唯尔公司All rights reserved.
Soy protein is reported to prevent bone loss in both women and rat models of osteoporosis. However, the role of soy isoflavones on the trabecular microarchitectural properties needs to be explored. In the present study, we examined whether soy protein with graded doses of isoflavones reverses loss of bone mineral density (BMD), bone mineral content (BMC), and trabecular microstructure in an ovariectomized (Ovx) osteopenic rat model. Seventy-eight 9-m old female Sprague-Dawley rats were either sham-operated (Sham; 1 group) or Ovx (5 groups) and fed a semi-purified casein-based diet. After 90 days, the occurrence of bone loss was confirmed using dual energy X-ray absorptiometry. Thereafter, rats were assigned to the following treatments: Sham, Ovx (control), Ovx + 17 beta-estradiol (E-2; 10 mu g/kg body wt. twice per week), Ovx + soy protein depleted of isoflavones (Soy-; 0.06 mg isoflavones/g protein), Ovx + soy protein with normal isoflavone content (Soy; 3.55 mg isoflavones/g protein), and Ovx + isoflavone-enriched soy protein (Soy+; 7.10 mg isoflavones/g protein). After 125 days of treatment, rats were euthanized, and tibia and lumbar bones were collected for the assessment of BMD, BMC, and trabecular rnicroarchitectural properties using X-ray microcomputed tomography. None of the treatments had an effect on BMD or microarchitectural properties of the lumbar vertebra. However, Soy treatment significantly increased tibial BMC and BMD by 10% and 4.5% compared with Ovx control, but the increase in BMD was not enough to reach the BMD levels of the Sham control group. The Soy(+) diet positively affected the tibial architectural properties including trabecular thickness, separation, and number. In summary, our findings suggest that soy protein does not restore bone loss in osteopenic rats; however, higher doses of isoflavones may be required to reverse the loss of tibial microstructural properties. (c) 2005 Elsevier Inc. All rights reserved.