Quantification and comparison of acidic polysaccharides in edible fish intestines and livers using HPLC-MS/MS
Quantification and comparison of acidic polysaccharides in edible fish intestines and livers using HPLC-MS/MS
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使用 HPLC-MS/MS 定量和比较食用鱼肠和肝脏中的酸性多糖
DOI：
10.1007/s10719-017-9783-6
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发表时间：
2017-07
影响因子：
3
通讯作者：
Wen Chengrong
中科院分区：
文献类型：
--
作者：
Song Shuang;Yu Qi;Zhang Bao;Ai Chunqing;Sun Yujiao;Fu Yinghuan;Zhao Meiyu;Wen Chengrong
在中国，鱼肠和鱼肝通常被认为是美味和营养丰富的食物。酸性多糖是这些动物源性食品中的重要营养素，但目前关于其数量分布的信息很少。本研究利用高效液相色谱-三重四极杆质谱联用分析酸水解产生的双糖同时定量酸性多糖的方法。这些酸性多糖的回收率均为97% ~ 115%，相对标准偏差为3.0% ~ 9.0%。所有酸性多糖均具有良好的线性关系。然后应用该方法测定了5种食用鱼肝脏和肠道中酸性多糖的组成。除了已知的透明质酸（HA）、硫酸软骨素（CS）、硫酸皮聚糖（DS）和肝素（HP）等糖胺聚糖外，还发现了4种新型酸性多糖，包括2种由己糖-己糖醛酸重复单元组成的糖胺聚糖和2种非糖胺聚糖。CS和HP分别是鱼肠道和肝脏中主要的酸性多糖成分。这些鱼类组织中酸性多糖的绝对含量差异很大，但它们的比例在同一类型的组织中表现出相似性。本研究建立了一种有效的酸性多糖定量方法，揭示了食用鱼肝脏和肠道中酸性多糖的组成。
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%–115% with relative standard deviation of 3.0%–9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines.
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影响因子：
2.6
作者：
Segarra S;Martínez-Subiela S;Cerdà-Cuéllar M;Martínez-Puig D;Muñoz-Prieto A;Rodríguez-Franco F;Rodríguez-Bertos A;Allenspach K;Velasco A;Cerón J
通讯作者：
Cerón J
影响因子：
6.5
作者：
Mourao, PAS;Guimaraes, MAM;Gray, E
通讯作者：
Gray, E
影响因子：
4.4
作者：
Steven A. Fazio;David C. White
通讯作者：
Steven A. Fazio;David C. White
影响因子：
5.3
作者：
du Souich P;García AG;Vergés J;Montell E
通讯作者：
Montell E
影响因子：
3.1
作者：
Wang, Hong-xu;Zhao, Jun;Zhang, Li-peng
通讯作者：
Zhang, Li-peng