Combining gellan gum with a functional low-molecular-weight gelator to assemble stiff shaped hybrid hydrogels for stem cell growth
Combining gellan gum with a functional low-molecular-weight gelator to assemble stiff shaped hybrid hydrogels for stem cell growth
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DOI：
10.1039/d2ma00565d
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发表时间：
2022-09-02
影响因子：
5
通讯作者：
Smith，David K.
中科院分区：
文献类型：
--
作者：
Piras，Carmen C.;Genever，Paul G.;Smith，David K.
我们报告了将结冷胶（GG）聚合物凝胶剂（PG）与基于1,3:2,4-二亚苄基山梨醇（DBS）的低分子量凝胶剂（LMWG）相结合的混合水凝胶。我们使用热-冷循环将这些凝胶制成珠子以凝固 LMWG 凝胶，然后使用不同的钙源（CaCl2 和 CaCO3）来交联结冷胶。对于 CaCO3，葡萄糖酸-δ-内酯 (GdL) 用作缓慢酸化剂，缓慢溶解钙离子并诱导 GG 交联。或者，光酸产生剂硝酸二苯基碘 (DPIN) 可以与紫外线照射一起使用，以溶解 CaCO3 并诱导 GG 凝胶化，在这种情况下，将光掩模应用于托盘中制成的凝胶可产生光图案化的凝胶。将 LMWG 与结冷胶结合进一步增强了 GG 的刚度，重要的是，使凝胶显着增强了对剪切应变的抵抗力。 LMWG/GG 混合凝胶比同等的 LMWG/藻酸盐凝胶坚硬得多。 DBS-CONHNH2 LMWG 保留了其将混合凝胶珠内的贵金属盐还原为纳米颗粒 (NP) 的独特能力，AgNP 的原位制造证明了这一点。混合凝胶珠可长时间支持人类间充质干细胞的生长。我们认为，这些混合凝胶良好的流变特性，加上 LMWG 原位形成 AgNP 的能力，可能会在未来的骨科应用中发挥潜在作用。
We report hybrid hydrogels that combine gellan gum (GG) polymer gelator (PG) with a low-molecular weight gelator (LMWG) based on 1,3:2,4-dibenzylidene sorbitol (DBS). We fabricate these gels into beads using a heat–cool cycle to set the LMWG gel and then using different calcium sources (CaCl2 and CaCO3) to subsequently crosslink the gellan gum. In the case of CaCO3, glucono-δ-lactone (GdL) is used as a slow acidification agent to slowly solubilise calcium ions and induce GG crosslinking. Alternatively the photoacid generator, diphenyliodonium nitrate (DPIN) can be used with UV irradiation to solubilise CaCO3 and induce GG gelation, in which case, a photomask applied to gels made in trays yields photopatterned gels. Combining the LMWG with gellan gum further enhances the stiffness of GG, and importantly, makes the gels significantly more resistant to shear strain. LMWG/GG hybrid gels are considerably stiffer than equivalent LMWG/alginate gels. The DBS-CONHNH2 LMWG retains its unique ability to reduce precious metal salts to nanoparticles (NPs) within the hybrid gel beads, as demonstrated by the in situ fabrication of AgNPs. The hybrid gel beads support the growth of human mesenchymal stem cells for extended periods of time. We suggest that the favourable rheological properties of these hybrid gels, combined with the ability of the LMWG to form AgNPs in situ, may enable potential future orthopedic applications.