Neuropeptide Y blocks GABAB-induced phase-shifts of the suprachiasmatic circadian clock in vitro.
Neuropeptide Y blocks GABAB-induced phase-shifts of the suprachiasmatic circadian clock in vitro.
复制标题
神经肽 Y 在体外阻断 GABAB 诱导的视交叉上生物钟的相移。
DOI：
10.1016/s0006-8993(99)01104-x
复制
发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Prosser,RA
中科院分区：
文献类型：
--
作者：
Biggs,KR;Prosser,RA
哺乳动物视交叉上核（SCN）中的生物钟在体外产生24小时的神经元活动节律。我们之前已经证明GABABagonist巴氯芬在体外以相位依赖的方式重置SCN起搏器：在授时数时间（ZT） 6时诱导提前，在ZT 22时诱导延迟。我们之前也表明，神经肽Y （NPY）在zt10应用时相移SCN时钟，而在其他时间则没有。在这里，我们发现NPY阻断了巴氯芬在zt6和zt22处引起的相移。NPY的抑制作用表现为剂量依赖性，并且需要更高浓度的NPY才能完全抑制巴氯芬诱导的相推进而不是相延迟。相反，在zt10处npy诱导的相移不受巴氯芬共应用的影响。这些结果与先前的研究结果一致，表明NPY在白天和夜间都能阻断多种神经调节剂诱导的体外相移。
The mammalian circadian clock in the suprachiasmatic nucleus (SCN) generates 24-h rhythms of neuronal activity in vitro. We have previously shown that the GABABagonist baclofen resets the SCN pacemaker in vitro in a phase-dependent manner: advances are induced at zeitgeber time (ZT) 6 and delays are induced at ZT 22. We have also previously shown that neuropeptide Y (NPY) phase-shifts the SCN clock when applied at ZT 10 but not at other times. Here, we show that NPY blocks the baclofen-induced phase-shifts at ZT 6 and ZT 22. The inhibition by NPY appears dose-dependent, and a higher concentration of NPY is required for complete inhibition of the baclofen-induced phase-advances than the phase-delays. Conversely, NPY-induced phase-shifts at ZT 10 are unaffected by co-application of baclofen. These results are consistent with previous findings showing that NPY blocks in vitro phase-shifts induced by a variety of neuromodulators during both the daytime and nighttime.