Infectious Clones of Novel Lineage 1 and Lineage 2 West Nile Virus Strains WNV-TX02 and WNV-Madagascar
Infectious Clones of Novel Lineage 1 and Lineage 2 West Nile Virus Strains WNV-TX02 and WNV-Madagascar
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DOI：
10.1128/jvi.00401-12
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发表时间：
2012-07-01
影响因子：
5.4
通讯作者：
Gale, Michael, Jr.
中科院分区：
文献类型：
--
作者：
Suthar, Mehul S.;Brassil, Margaret M.;Gale, Michael, Jr.
我们报告西尼罗河病毒（WNV）感染性克隆的致病性谱系1德州HC 2002和非致病性谱系2马达加斯加AnMg 798株。感染性克隆表现出与亲本病毒分离株相似的生物学特性。我们产生了嵌合病毒，并发现WNV-TX的结构和非结构区域内的病毒因子有助于控制I型干扰素防御。这些感染性克隆为研究黄病毒的免疫调节和致病机制提供了新的试剂。
We report the generation of West Nile virus (WNV) infectious clones for the pathogenic lineage 1 Texas-HC2002 and nonpathogenic lineage 2 Madagascar-AnMg798 strains. The infectious clones exhibited biological properties similar to those of the parental virus isolates. We generated chimeric viruses and found that viral factors within the structural and nonstructural regions of WNV-TX contribute to the control of type I interferon defenses. These infectious clones provide new reagents to study flavivirus immune regulation and pathogenesis.