RHO-PROTEIN REGULATES TIGHT JUNCTIONS AND PERIJUNCTIONAL ACTIN ORGANIZATION IN POLARIZED EPITHELIA
RHO-PROTEIN REGULATES TIGHT JUNCTIONS AND PERIJUNCTIONAL ACTIN ORGANIZATION IN POLARIZED EPITHELIA
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DOI：
10.1073/pnas.92.23.10629
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发表时间：
1995-11-07
影响因子：
11.1
通讯作者：
MADARA, JL
中科院分区：
文献类型：
--
作者：
NUSRAT, A;GIRY, M;MADARA, JL
GTP结合蛋白的rho家族调节肌动蛋白丝组织。在非极化的哺乳动物细胞中，rho蛋白调节细胞-基质界面处含肌动蛋白的应力纤维的组装。相反，极化上皮细胞是高的和圆柱形的，具有发达的细胞间紧密连接，使它们能够充当生物屏障。我们报告说，rho调节丝状肌动蛋白组织优先在极化肠上皮细胞的顶端，这样做，影响相关的顶端紧密连接的组织和渗透性。因此，屏障功能，这是柱状上皮细胞的基本特征，是由rho调节。
The rho family of GTP-binding proteins regulates actin filament organization. In unpolarized mammalian cells, rho proteins regulate the assembly of actin-containing stress fibers at the cell-matrix interface. Polarized epithelial cells, in contrast, are tall and cylindrical with well developed intercellular tight junctions that permit them to behave as biologic barriers. We report that rho regulates filamentous actin organization preferentially in the apical pole of polarized intestinal epithelial cells and, in so doing, influences the organization and permeability of the associated apical tight junctions. Thus, barrier function, which is an essential characteristic of columnar epithelia, is regulated by rho.