Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin
Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin
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DOI：
10.1016/s0006-3495(03)74501-9
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发表时间：
2003-07-01
影响因子：
3.4
通讯作者：
Puppels, GJ
中科院分区：
文献类型：
--
作者：
Caspers, PJ;Lucassen, GW;Puppels, GJ
体内共焦拉曼光谱是一种非侵入性光学方法，能够以高空间分辨率获取有关皮肤分子组成的详细信息。体内共焦扫描激光显微镜是一种成像方式，无需物理解剖组织即可提供皮肤的光学切片。描述了在单个仪器中两种技术的组合。这种组合可以使皮肤形态可视化，并且可以将皮肤中的（表面下）结构作为拉曼测量的目标。提出的新颖结果通过皮肤的体内横截面图像显示了与皮肤结构直接相关的角质层的水和天然保湿因子的详细体内浓度分布。通过原位记录汗管和皮脂腺的体内拉曼光谱来证明皮肤结构的靶向性。对真皮毛细血管的体内测量以完全无创的方式产生了高质量的血液拉曼光谱。根据这项探索性研究的结果，我们得出结论，所提出的技术在基础皮肤研究、药理学（经皮转运）、临床皮肤病学和化妆品研究以及血液分析物（包括葡萄糖）的无创分析方面具有巨大潜力。
In vivo confocal Raman spectroscopy is a noninvasive optical method to obtain detailed information about the molecular composition of the skin with high spatial resolution. In vivo confocal scanning laser microscopy is an imaging modality that provides optical sections of the skin without physically dissecting the tissue. A combination of both techniques in a single instrument is described. This combination allows the skin morphology to be visualized and (subsurface) structures in the skin to be targeted for Raman measurements. Novel results are presented that show detailed in vivo concentration profiles of water and of natural moisturizing factor for the stratum corneum that are directly related to the skin architecture by in vivo cross-sectional images of the skin. Targeting of skin structures is demonstrated by recording in vivo Raman spectra of sweat ducts and sebaceous glands in situ. In vivo measurements on dermal capillaries yielded high-quality Raman spectra of blood in a completely noninvasive manner. From the results of this exploratory study we conclude that the technique presented has great potential for fundamental skin research, pharmacology (percutaneous transport), clinical dermatology, and cosmetic research, as well as for noninvasive analysis of blood analytes, including glucose.