Mapping of the K+/Na+ discrimination locus Kna1 in wheat
Mapping of the K+/Na+ discrimination locus Kna1 in wheat
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DOI：
10.1007/bf00223692
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发表时间：
1996-03-01
影响因子：
5.4
通讯作者：
Dvorak, J
中科院分区：
文献类型：
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作者：
Dubcovsky, J;Maria, GS;Dvorak, J
在盐分环境中，面包小麦 Triticum aestivum L.（基因组 AABBDD）在展开叶和幼叶中比硬粒小麦 T. turgidum L.（基因组 AABB）积累更少的 Na+ 和更多的 K+。面包小麦叶子中较高的 K+/Na+ 比率与其较高的耐盐性相关。之前的研究表明，面包小麦的 4D 染色体在控制这一性状方面发挥着重要作用，并在缺乏 Phl 位点的情况下与 4B 染色体重组。在 T. turgidum 遗传背景下开发了 4D/4B 重组染色体二体植物群体，以研究 4D 染色体对 K+/Na+ 辨别的遗传控制。有证据表明，该性状是由 4D 染色体长臂上的单个基因座（称为 Kna1）控制的。在目前的工作中，针对具有 4D/4B 染色体的其他家族确定了 K+/Na+ 歧视。最年轻叶片中的 Na+ 浓度和 K+/Na+ 比率聚集在两个不重叠的类别中，并且所有重组家族都可以明确地分配给 Kna1 和 kan1 类别。以这种方式评分的 Kna1 基因座被映射到 4DL 臂中的一个短区域上，并与 Xwg199、Xabc305、Xbcd402、Xpsr567 和 Xpsr375 完全连锁；它还被映射为数量性状。基于温室种植植物幼叶和田间种植植物剑叶中 K+/Na+ 比率的 QTL 分析结果与确定为质量性状的 Kna1 的位置一致。讨论了通过操纵 Phl 基因座进行基因渗入的几个方面。
In saline environments, bread wheat, Triticum aestivum L. (genomes AABBDD), accumulates less Na+ and more K+ in expanding and young leaves than durum wheat, T. turgidum L. (genomes AABB). Higher K+/Na+ ratios in leaves of bread wheat correlate with its higher salt tolerance. Chromosome 4D from bread wheat was shown in previous work to play an important role in the control of this trait and was recombined with chromosome 4B in the absence of the Phl locus. A population of plants disomic for 4D/4B recombined chromosomes in the genetic background of T. turgidum was developed to investigate the genetic control of K+/Na+ discrimination by chromosome 4D. Evidence was obtained that the trait is controlled by a single locus, designated Kna1, in the long arm of chromosome 4D. In the present work, K+/Na+ discrimination was determined for additional families with 4D/4B chromosomes. The concentrations of Na+ and K+/Na+ ratios in the youngest leaf blades clustered in two nonoverlapping classes, and all recombinant families could be unequivocally assigned to Kna1 and kan1 classes. The Kna1 locus scored this way was mapped on a short region in the 4DL arm and was completely linked to Xwg199, Xabc305, Xbcd402, Xpsr567, and Xpsr375; it was also mapped as a quantitative trait. The results of the QTL analysis, based on the K+/Na+ ratios in the young leaves of greenhouse-grown plants and flag leaves of field-grown plants, agreed with the position of Kna1 determined as a qualitative trait. Several aspects of gene introgression by manipulation of the Phl locus are discussed.