Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection
Comparative proteomic analysis of differentially expressed proteins in the Bombyx mori fat body during the microsporidia Nosema bombycis infection
复制标题
家蚕微孢子虫感染过程中家蚕脂肪体差异表达蛋白的比较蛋白质组学分析
DOI：
10.1016/j.jip.2017.06.009
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发表时间：
2017
影响因子：
3.4
通讯作者：
Zhou ZeYang
中科院分区：
文献类型：
--
作者：
Li ZhiHong;Pan GuoQing;Ma ZhenGang;Han Bing;Sun Bin;Ni Qi;Chen Jie;Li Tian;Liu TongBao;Long MengXian;Li ChunFeng;Zhou ZeYang
家蚕微孢子虫是一种专性的细胞内寄生虫，可引起海水病。调查……的影响。家蚕感染，5龄家蚕用N。用Bombycisisolate CQ1和双向凝胶电泳法分析感染和未感染家蚕脂肪体在感染后1、2、4、6和8天(Dpi)的差异表达蛋白质。利用MALDI-TOF/TOF MS技术在5个时间点鉴定出46个差异表达蛋白质，这些蛋白质主要涉及免疫应答、能量代谢和分子合成。总体而言，已识别的蛋白质可能为了解家蚕响应TON的机制提供重要的见解。炸弹顺式感染。
Nosema bombycisis an obligate intracellular parasite, which can cause pébrine disease. To investigate the effects ofN. bombycisinfection, 5th-instar silkworms were challenged withN. bombycisisolate CQ1, and two-dimensional gel electrophoresis analysis was performed to analyze the differentially expressed proteins in infected and uninfected silkworm fat bodies 1, 2, 4, 6 and 8 days post-infection (dpi). 46 differentially expressed proteins were identified at the 5 time points using MALDI-TOF/TOF MS. The changed proteins mainly involved in immune response, energy metabolism, and molecular synthesis. Overall, the identified proteins may provide important insights into the mechanisms of the silkworm response toN. bombycisinfection.